Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041127_0k6o GCCTCCAACTCCCATTGACAGCCTCAATGAGG(+ATATAT)GTACCATACATCATGAGATAAGTATGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240607041125_utuo ACATGTTCTTCATACTTATCTCATGATGTATG(+ATATAT)GTACCCTCATTGAGGCTGTCAATGGGAGTTG NGG 10 8 17 8 24 0 100 5 10
    240607041124_1uv7 TTTTGTATTTATTTGTATGTTCAGATCCAAAG(+ATATAT)GATCCGTTGTAAAAATATATATGTGAATATA NGG 10 8 17 8 24 0 100 5 10
    240607041122_x8jc GTATATATATTCACATATATATTTTTACAACG(+ATATAT)GATCCTTTGGATCTGAACATACAAATAAATA NGG 10 8 17 8 24 0 100 5 10
    240607041121_uldo TGGCATACTTATGCCATATCTGTTTTGATTGG(+ATATAT)GATCCTGGAACCTCCCTTAAATCAAGGATTG NGG 10 8 17 8 24 0 100 5 10
    240607041119_rrsq AAGGCCAATCCTTGATTTAAGGGAGGTTCCAG(+ATATAT)GATCCCAATCAAAACAGATATGGCATAAGTA NGG 10 8 17 8 24 0 100 5 10
    240607041117_f9s8 GCCACTTCTGAGCTGAGCACCTGTCTGAGGAG(+ATATAT)GATCCTGACTAGACAAGGAATTCATAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607041116_ux2c CTCTCTGGCTTATGAATTCCTTGTCTAGTCAG(+ATATAT)GATCCTCCTCAGACAGGTGCTCAGCTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240607041115_svm4 GGAGATGTGTCTCTGGGTGGATGAGCTATTTG(+ATATAT)GTACCTATAACCTGTGTGGCTCTGTAATATA NGG 10 8 17 8 24 0 100 5 10
    240607041114_3yc8 CCCCTTATATTACAGAGCCACACAGGTTATAG(+ATATAT)GTACCAAATAGCTCATCCACCCAGAGACACA NGG 10 8 17 8 24 0 100 5 10