Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041058_piah TGTATTCGTTGTATGTGTGTACACACAGTCTG(+ATATAT)GTACCTGACATTCAACAGTGACTCTTTATAG NGG 10 8 17 8 24 0 100 5 10
    240607041057_kqdb CCAGGCTATAAAGAGTCACTGTTGAATGTCAG(+ATATAT)GTACCAGACTGTGTGTACACACATACAACGA NGG 10 8 17 8 24 0 100 5 10
    240607041056_zvy0 ATAGTGCCCCTGTGGCCCAGAAGAGAGTGTTG(+ATATAT)GATCCCTGAGACTGGAGTTATAGACAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240607041054_549u AGTTCACTTCTGTCTATAACTCCAGTCTCAGG(+ATATAT)GATCCAACACTCTCTTCTGGGCCACAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240607041053_rcl1 TCTGGTTAGTATAAGTCAAATCAGGGTATTTG(+ATATAT)GTACCTGTCTTGCTGTACTGAATTATAGCTA NGG 10 8 17 8 24 0 100 5 10
    240607041052_eega ATTATTAGCTATAATTCAGTACAGCAAGACAG(+ATATAT)GTACCAAATACCCTGATTTGACTTATACTAA NGG 10 8 17 8 24 0 100 5 10
    240607041051_lf9a CACTGCCTGTGCTGCTAGGATTTAGTTATAAG(+ATATAT)GATCCAGTACTCCAGGGCCAGGGCCAGTGTA NGG 10 8 17 8 24 0 100 5 10
    240607041050_b5rm CTCTGTACACTGGCCCTGGCCCTGGAGTACTG(+ATATAT)GATCCTTATAACTAAATCCTAGCAGCACAGG NGG 10 8 17 8 24 0 100 5 10
    240607041048_f1nt TGAATTGGGATTACTTTGATATTTTATTTTGG(+ATATAT)GATCCTGTGCCACAAAATGACATGTGTAATG NGG 10 8 17 8 24 0 100 5 10
    240607041047_rz8d GTAAACATTACACATGTCATTTTGTGGCACAG(+ATATAT)GATCCCAAAATAAAATATCAAAGTAATCCCA NGG 10 8 17 8 24 0 100 5 10