Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041019_0zqn AACTGCCATTCTCTGAAGACAGAGCAACAGGG(+ATATAT)GTACCCCTGCCCCAGGGCTGCTCTCCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240607041017_7m61 GAGGGCCAAGGGAGAGCAGCCCTGGGGCAGGG(+ATATAT)GTACCCCTGTTGCTCTGTCTTCAGAGAATGG NGG 10 8 17 8 24 0 100 5 10
    240607041016_sadr CCAGAGAGCTCTGAGAGAGGACAAGGCAGCTG(+ATATAT)GTACCCTGACCAATGTCCTCAGCCACACACA NGG 10 8 17 8 24 0 100 5 10
    240607041014_06b5 GAGGGTGTGTGTGGCTGAGGACATTGGTCAGG(+ATATAT)GTACCAGCTGCCTTGTCCTCTCTCAGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240607041012_o06i GGGGACCCAATTCTTTCTTCTGGCCTCCAAGG(+ATATAT)GTACCAGCCATACATACATACATACATACAT NGG 10 8 17 8 24 0 100 5 10
    240607041010_n5fq TATGTATGTATGTATGTATGTATGTATGGCTG(+ATATAT)GTACCCTTGGAGGCCAGAAGAAAGAATTGGG NGG 10 8 17 8 24 0 100 5 10
    240607041009_gck7 CCCAACCCCTCTAAACCAGCTCTGTGGCTCAG(+ATATAT)GTACCTTCAGTGAAGCCCATTGCTCTTGCCG NGG 10 8 17 8 24 0 100 5 10
    240607041008_men8 CTGGGCGGCAAGAGCAATGGGCTTCACTGAAG(+ATATAT)GTACCTGAGCCACAGAGCTGGTTTAGAGGGG NGG 10 8 17 8 24 0 100 5 10
    240607041006_z3vf GAATGAGATTTTGTATTTTCCCTTGAGAGATG(+ATATAT)GTACCAAGCAGGCAAGCAACCATGTACCTAC NGG 10 8 17 8 24 0 100 5 10
    240607041005_8am6 AACTGGTAGGTACATGGTTGCTTGCCTGCTTG(+ATATAT)GTACCATCTCTCAAGGGAAAATACAAAATCT NGG 10 8 17 8 24 0 100 5 10