Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040919_slbu AGTAAGGAGGGGACTGAGTACGTAACCTGTTG(+ATATAT)GTACCCTTAAACTGGACAAACGTCACAATGC NGG 10 8 17 8 24 0 100 5 10
    240607040918_2p5t ATGTGGCATTGTGACGTTTGTCCAGTTTAAGG(+ATATAT)GTACCAACAGGTTACGTACTCAGTCCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240607040916_t89l TACTGACCAGTTCAGATGGGTCCACTAGAGGG(+ATATAT)GATCCTTGAGCATGCACACTTGGAAAAACAC NGG 10 8 17 8 24 0 100 5 10
    240607040915_1rdb CATGGGTGTTTTTCCAAGTGTGCATGCTCAAG(+ATATAT)GATCCCCTCTAGTGGACCCATCTGAACTGGT NGG 10 8 17 8 24 0 100 5 10
    240607040914_1z1a TGAGCTTAGCTTGTGCTGGCGGCTGAACTCAG(+ATATAT)GATCCTGTCCTGCACAGGCTAGGCAGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240607040912_d7k5 GGTAGAGGGCCTGCCTAGCCTGTGCAGGACAG(+ATATAT)GATCCTGAGTTCAGCCGCCAGCACAAGCTAA NGG 10 8 17 8 24 0 100 5 10
    240607040911_2e5g TAATACCAACATTCACATACTGTATTACAATG(+ATATAT)GTACCACAATATAAAATAAAATATGTATAAA NGG 10 8 17 8 24 0 100 5 10
    240607040910_br9p GTCAGTTTATACATATTTTATTTTATATTGTG(+ATATAT)GTACCATTGTAATACAGTATGTGAATGTTGG NGG 10 8 17 8 24 0 100 5 10
    240607040908_dusv CCCATAGGCACAAGGCGAGGGATGGTCCCAAG(+ATATAT)GATCCGTAAGACAGTGTTTGCCATCCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240607040907_mtuw ACCAGACAGAGGATGGCAAACACTGTCTTACG(+ATATAT)GATCCTTGGGACCATCCCTCGCCTTGTGCCT NGG 10 8 17 8 24 0 100 5 10