Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041004_fr96 AATTGGGTTTTGTCTTCTTTAAAAACAAATAG(+ATATAT)GTACCGTAAGCATTTTCTGCTGACGACTGCC NGG 10 8 17 8 24 0 100 5 10
    240607041003_6z4b CTTAAGGCAGTCGTCAGCAGAAAATGCTTACG(+ATATAT)GTACCTATTTGTTTTTAAAGAAGACAAAACC NGG 10 8 17 8 24 0 100 5 10
    240607041001_arhj TGAATAAGAACCAAACTGAGTATTATAGTGAG(+ATATAT)GATCCTTGACCACTTAGTAGACCTCAGCAAA NGG 10 8 17 8 24 0 100 5 10
    240607041000_v0n2 AGAATTTTGCTGAGGTCTACTAAGTGGTCAAG(+ATATAT)GATCCTCACTATAATACTCAGTTTGGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240607040958_abx8 ACCTTCGCAAAAACTTAAATCATGGAATGGCG(+ATATAT)GTACCACGGTAGAACACTGCAGCTGTCGCCA NGG 10 8 17 8 24 0 100 5 10
    240607040957_670z AACCCTGGCGACAGCTGCAGTGTTCTACCGTG(+ATATAT)GTACCGCCATTCCATGATTTAAGTTTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240607040954_uk81 GAATCGACCACCATACCACAGTAACGCCCCTG(+ATATAT)GATCCCTGGCTGCCCACCCACTCTAAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240607040952_falq CAGGATAGCCTTAGAGTGGGTGGGCAGCCAGG(+ATATAT)GATCCAGGGGCGTTACTGTGGTATGGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240607040950_ytw8 TACAAACACTAAGCATAAAAGCACATGTGGTG(+ATATAT)GTACCTGGGTATCTAGTTCTTACGGAATAAT NGG 10 8 17 8 24 0 100 5 10
    240607040948_3sgd TTGAGATTATTCCGTAAGAACTAGATACCCAG(+ATATAT)GTACCACCACATGTGCTTTTATGCTTAGTGT NGG 10 8 17 8 24 0 100 5 10