Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040933_gyri ACCACATGGTGGCTCACAACCATCTGTAATAG(+ATATAT)GATCCAATGCACTTTTCTGGTGTGTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240607040932_q141 TGTCTTCAGACACACACCAGAAAAGTGCATTG(+ATATAT)GATCCTATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607040930_cpbt ATCACACCACCATTTGTGCCTCCATCCCAGTG(+ATATAT)GATCCAACACCAACCTCTGACCTCCACAGGA NGG 10 8 17 8 24 0 100 5 10
    240607040929_omer GAAGTTCCTGTGGAGGTCAGAGGTTGGTGTTG(+ATATAT)GATCCACTGGGATGGAGGCACAAATGGTGGT NGG 10 8 17 8 24 0 100 5 10
    240607040927_1lnd CAGGAGTCAGCTCTTTTTCTTGGGAGTAAGAG(+ATATAT)GTACCAAAATAAGCAGAAAACAAAAAAATCA NGG 10 8 17 8 24 0 100 5 10
    240607040926_vvi9 TTAGTTGATTTTTTTGTTTTCTGCTTATTTTG(+ATATAT)GTACCTCTTACTCCCAAGAAAAAGAGCTGAC NGG 10 8 17 8 24 0 100 5 10
    240607040924_no0a ACCTCTGTGATGGGCTGCTTGTCCGGGACCAG(+ATATAT)GTACCATGCTTAGGCTAAGTGCATGATGTCA NGG 10 8 17 8 24 0 100 5 10
    240607040923_ahzd CACAGTGACATCATGCACTTAGCCTAAGCATG(+ATATAT)GTACCTGGTCCCGGACAAGCAGCCCATCACA NGG 10 8 17 8 24 0 100 5 10
    240607040922_r150 ACAGCATTCCGAGTACATGGACACTACATACG(+ATATAT)GTACCAGCTCCCTGGAAAAGATAAATATTGG NGG 10 8 17 8 24 0 100 5 10
    240607040921_umh0 TCGATCCAATATTTATCTTTTCCAGGGAGCTG(+ATATAT)GTACCGTATGTAGTGTCCATGTACTCGGAAT NGG 10 8 17 8 24 0 100 5 10