Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040947_yq5k GAAGAAGAATCTTGAAAGGGAGGGTGGTGATG(+ATATAT)GTACCGATAAATACCTGTGTTTTAAAATCTT NGG 10 8 17 8 24 0 100 5 10
    240607040946_lu5n TGTACAAGATTTTAAAACACAGGTATTTATCG(+ATATAT)GTACCATCACCACCCTCCCTTTCAAGATTCT NGG 10 8 17 8 24 0 100 5 10
    240607040944_r6ue TTTAGAATTCAGAGCTAGGTGGGGCTGATGGG(+ATATAT)GATCCTCTGATCCCGTCTGCCTTGATGGAAG NGG 10 8 17 8 24 0 100 5 10
    240607040943_pu9o GTGCTCTTCCATCAAGGCAGACGGGATCAGAG(+ATATAT)GATCCCCATCAGCCCCACCTAGCTCTGAATT NGG 10 8 17 8 24 0 100 5 10
    240607040941_zlbk AAAAGAATATACCTTCTTCTCAGCACCTCATG(+ATATAT)GTACCTTCTCCAAAATTGACCATATAATCGG NGG 10 8 17 8 24 0 100 5 10
    240607040940_22ts TGTGACCGATTATATGGTCAATTTTGGAGAAG(+ATATAT)GTACCATGAGGTGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240607040938_kzux CCTCTTCCAAACGACCCTAGTTCAATTTCCAG(+ATATAT)GATCCACATGGCAACTCATAGATGTCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240607040937_dmzi TGAAGTTACAGACATCTATGAGTTGCCATGTG(+ATATAT)GATCCTGGAAATTGAACTAGGGTCGTTTGGA NGG 10 8 17 8 24 0 100 5 10
    240607040936_dfy0 TCAGGGCAGAGAAACTGAGGAGCCTGGAGAAG(+ATATAT)GATCCCTCCGGTTCCCATCTGTGCCTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240607040934_ent6 GGTCAGCTCCAGGCACAGATGGGAACCGGAGG(+ATATAT)GATCCTTCTCCAGGCTCCTCAGTTTCTCTGC NGG 10 8 17 8 24 0 100 5 10