Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040819_hnxl ATAAAGAAATGTAAACAGTTTAACACCGACAG(+ATATAT)GATCCCTCTGCTAGTCCTTATTGTGACTGTT NGG 10 8 17 8 24 0 100 5 10
    240607040818_rwdo TCTGGAACAGTCACAATAAGGACTAGCAGAGG(+ATATAT)GATCCTGTCGGTGTTAAACTGTTTACATTTC NGG 10 8 17 8 24 0 100 5 10
    240607040817_z5lx TGGTGGAGGAAGTTCAGACCACACCTGAACTG(+ATATAT)GTACCTGAGATGACAGCACTGAATCTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240607040815_0j7m CTTTGTCTTCAGATTCAGTGCTGTCATCTCAG(+ATATAT)GTACCAGTTCAGGTGTGGTCTGAACTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240607040813_ylzz CCAGTGTCTGAGGCCGCCAGGGAGGTGCGGGG(+ATATAT)GTACCCCACATCCCCACGAGGCCTCATCTGC NGG 10 8 17 8 24 0 100 5 10
    240607040811_v1tf ATAGTGCAGATGAGGCCTCGTGGGGATGTGGG(+ATATAT)GTACCCCCGCACCTCCCTGGCGGCCTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240607040810_cqvb TTGTACAGCTGCGAGGGAGGGCACAGATGCAG(+ATATAT)GTACCTGCGCACTCCGCCCTTCTCTCTTACA NGG 10 8 17 8 24 0 100 5 10
    240607040809_wgyk GGGTGTGTAAGAGAGAAGGGCGGAGTGCGCAG(+ATATAT)GTACCTGCATCTGTGCCCTCCCTCGCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240607040807_fzrw TTCTAGGCACACTCCTTGCAGTAGGCTCCAGG(+ATATAT)GTACCTTGGAACTGCAACCAAGGCATGGTAA NGG 10 8 17 8 24 0 100 5 10
    240607040805_qu0f ATTCCTTACCATGCCTTGGTTGCAGTTCCAAG(+ATATAT)GTACCCTGGAGCCTACTGCAAGGAGTGTGCC NGG 10 8 17 8 24 0 100 5 10