Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040720_ewsu ACATGTAAATTAATATTTAGAGTGAAGGAATG(+ATATAT)GATCCAATTGATGAAGGAAGATGAGGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240607040719_p914 TGATAGGCTTCCTCATCTTCCTTCATCAATTG(+ATATAT)GATCCATTCCTTCACTCTAAATATTAATTTA NGG 10 8 17 8 24 0 100 5 10
    240607040718_ccc5 ACCGTTTCGCTAATAGTTACTGCCAGCAGCTG(+ATATAT)GATCCTGCATACAAAACACTTGTTCATGTTT NGG 10 8 17 8 24 0 100 5 10
    240607040716_8ur0 AAGGAAAACATGAACAAGTGTTTTGTATGCAG(+ATATAT)GATCCAGCTGCTGGCAGTAACTATTAGCGAA NGG 10 8 17 8 24 0 100 5 10
    240607040715_a63i GTCTGCAGTGGGCAGGGTGCTGGAGGAGAAGG(+ATATAT)GATCCAGGCAGACCCCACTCCAACTGAACCA NGG 10 8 17 8 24 0 100 5 10
    240607040713_n38h GTATGTGGTTCAGTTGGAGTGGGGTCTGCCTG(+ATATAT)GATCCCTTCTCCTCCAGCACCCTGCCCACTG NGG 10 8 17 8 24 0 100 5 10
    240607040711_f99p GCATTTTTTTTCCATAGGTAACTAACACCATG(+ATATAT)GATCCAACCAGGCAGAAGTTTGGAGCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240607040710_ubkn AGCAAAACTTGCTCCAAACTTCTGCCTGGTTG(+ATATAT)GATCCATGGTGTTAGTTACCTATGGAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607040708_yayg AGACCCTATACAAGCAGCATCGCTGTTGCCAG(+ATATAT)GATCCTCTTCCTAATGAAGGGTCGCAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240607040706_qpfj ACAGCGTCTCTGCGACCCTTCATTAGGAAGAG(+ATATAT)GATCCTGGCAACAGCGATGCTGCTTGTATAG NGG 10 8 17 8 24 0 100 5 10