Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040633_irun GGCCTCCCATGGGTTTGGAGGATTTGAACTTG(+ATATAT)GATCCTTACACCGTTGTGGCAAGCACTTTGC NGG 10 8 17 8 24 0 100 5 10
    240607040631_cuvz TCAGGGCAAAGTGCTTGCCACAACGGTGTAAG(+ATATAT)GATCCAAGTTCAAATCCTCCAAACCCATGGG NGG 10 8 17 8 24 0 100 5 10
    240607040630_mwdu CCTAAATCAAAGGCTGTCAGCTGCTGGGCAAG(+ATATAT)GATCCTACAAGGCAGGGACTGGGCCCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240607040629_bf4i AGGTGACACAGGGCCCAGTCCCTGCCTTGTAG(+ATATAT)GATCCTTGCCCAGCAGCTGACAGCCTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240607040628_8c4y GGGCCCTAGGTGGTCAGTCACCTCAGGGCTGG(+ATATAT)GTACCTGCTAAGGGAGACCCAGGTGGTTCAC NGG 10 8 17 8 24 0 100 5 10
    240607040627_2zgx CCTACGTGAACCACCTGGGTCTCCCTTAGCAG(+ATATAT)GTACCCAGCCCTGAGGTGACTGACCACCTAG NGG 10 8 17 8 24 0 100 5 10
    240607040625_uql8 GGCGTCCCCGTCTGCTGCAGAGGGGACACTCG(+ATATAT)GATCCAGCCTGTGGCCACCCCGGCAATAGAC NGG 10 8 17 8 24 0 100 5 10
    240607040624_yxcg GTGGTGTCTATTGCCGGGGTGGCCACAGGCTG(+ATATAT)GATCCGAGTGTCCCCTCTGCAGCAGACGGGG NGG 10 8 17 8 24 0 100 5 10
    240607040622_gqn7 TTGCCTGCAATTCCTGTGGTGTCCACCAGGGG(+ATATAT)GATCCTAGCACTGTGCCTGCTACAGAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240607040621_zfdx CTGAGGCCCTTCTGTAGCAGGCACAGTGCTAG(+ATATAT)GATCCCCCTGGTGGACACCACAGGAATTGCA NGG 10 8 17 8 24 0 100 5 10