Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040604_ajrh CCAGCTCCCTCACAGGGGGCTGGGGGGAGGGG(+ATATAT)GATCCTCAACAAGGACTCCACCCTCACCATG NGG 10 8 17 8 24 0 100 5 10
    240607040603_sn5u GTGGGCATGGTGAGGGTGGAGTCCTTGTTGAG(+ATATAT)GATCCCCCTCCCCCCAGCCCCCTGTGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240607040601_ai5r GGGCGCGTTTGTTCCTCACCCACGCGCGTGGG(+ATATAT)GATCCCCTCGCCACAGGGAAGCACAAGAACC NGG 10 8 17 8 24 0 100 5 10
    240607040559_pbn3 GAGGGGGTTCTTGTGCTTCCCTGTGGCGAGGG(+ATATAT)GATCCCCACGCGCGTGGGTGAGGAACAAACG NGG 10 8 17 8 24 0 100 5 10
    240607040558_4vqi TCAAGTAGACGACATCAGGGTCTGATATCAAG(+ATATAT)GATCCAGTCCCTCTTCAGGGCTTATGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240607040557_elub AAGTGCACCCCATAAGCCCTGAAGAGGGACTG(+ATATAT)GATCCTTGATATCAGACCCTGATGTCGTCTA NGG 10 8 17 8 24 0 100 5 10
    240607040555_kit5 GAAAAGAAGATCCAGTGACAAGTCCAAAATGG(+ATATAT)GATCCAGCTCAAAGGGAGGTCCCAAGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240607040554_m5cd AGTGTCAGGCCTTGGGACCTCCCTTTGAGCTG(+ATATAT)GATCCCATTTTGGACTTGTCACTGGATCTTC NGG 10 8 17 8 24 0 100 5 10
    240607040553_6dnl AATAATACACTTGAGGAATGGTTCTGTGAATG(+ATATAT)GTACCTTTCAATATTTTGGTTATTGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240607040552_6543 ATATTAACAAACAATAACCAAAATATTGAAAG(+ATATAT)GTACCATTCACAGAACCATTCCTCAAGTGTA NGG 10 8 17 8 24 0 100 5 10