Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040704_t9ia CAGTATTACCATTATAAATGCTATCCTCCAGG(+ATATAT)GATCCGCTGCTCCCACTAGCATAAGCCTTCA NGG 10 8 17 8 24 0 100 5 10
    240607040703_hygw CAAAGTGAAGGCTTATGCTAGTGGGAGCAGCG(+ATATAT)GATCCCTGGAGGATAGCATTTATAATGGTAA NGG 10 8 17 8 24 0 100 5 10
    240607040702_kv5b AGTCCTCTGGAAATTCAGATGAAAGGCGTGAG(+ATATAT)GTACCAAAAATAATAATTTGTCAATTTAGTT NGG 10 8 17 8 24 0 100 5 10
    240607040700_ccih TCCACAACTAAATTGACAAATTATTATTTTTG(+ATATAT)GTACCTCACGCCTTTCATCTGAATTTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240607040659_4s8z GCACTATGTTGACCAAGCCTGCCAGCCCTATG(+ATATAT)GTACCTTTCTTTAAATGGCCGAATCCCTGGT NGG 10 8 17 8 24 0 100 5 10
    240607040657_lj3q TACTAACCAGGGATTCGGCCATTTAAAGAAAG(+ATATAT)GTACCATAGGGCTGGCAGGCTTGGTCAACAT NGG 10 8 17 8 24 0 100 5 10
    240607040656_d0v1 TGCAGCAAGTCATCTAATGCATGTGCACTTAG(+ATATAT)GATCCAAGGACAAGAAAAGAACAGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240607040655_jn2j TGTTTCTCTCTCCTGTTCTTTTCTTGTCCTTG(+ATATAT)GATCCTAAGTGCACATGCATTAGATGACTTG NGG 10 8 17 8 24 0 100 5 10
    240607040653_039a CCCCAGTGAGGAGTTAGGGAATGGTTGTGGTG(+ATATAT)GATCCCCTGCCTAGAATCTCCCAGTGAGGCG NGG 10 8 17 8 24 0 100 5 10
    240607040651_fr6j CTGAGCGCCTCACTGGGAGATTCTAGGCAGGG(+ATATAT)GATCCACCACAACCATTCCCTAACTCCTCAC NGG 10 8 17 8 24 0 100 5 10