Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040551_bg52 CTTACCATCACAGTAAGACATAAGTGGGTGAG(+ATATAT)GATCCCTTCTGTCATTGATTCTTTCTGTAAA NGG 10 8 17 8 24 0 100 5 10
    240607040549_d7rz CACAGTTTACAGAAAGAATCAATGACAGAAGG(+ATATAT)GATCCTCACCCACTTATGTCTTACTGTGATG NGG 10 8 17 8 24 0 100 5 10
    240607040548_ezk1 TCAAAGGCTCAATGCTGCCCTAAATTAAACAG(+ATATAT)GTACCATGGGCAGTCTAAGTGGGTATTGTGC NGG 10 8 17 8 24 0 100 5 10
    240607040545_dz0w CTCAGGCACAATACCCACTTAGACTGCCCATG(+ATATAT)GTACCTGTTTAATTTAGGGCAGCATTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240607040543_uggb TGTGGGAGCAGGTTTCCTGTAGAGCGTTCCAG(+ATATAT)GTACCACAAGATACAGGGCTCCTATTCAACC NGG 10 8 17 8 24 0 100 5 10
    240607040542_ga32 TTGGGGGTTGAATAGGAGCCCTGTATCTTGTG(+ATATAT)GTACCTGGAACGCTCTACAGGAAACCTGCTC NGG 10 8 17 8 24 0 100 5 10
    240607040541_z4ux AGTTTGACTATAAGAATTTCACTTCTCAACAG(+ATATAT)GTACCTGAACAGTACAATATTAACGATATTG NGG 10 8 17 8 24 0 100 5 10
    240607040540_jilm AAGTACAATATCGTTAATATTGTACTGTTCAG(+ATATAT)GTACCTGTTGAGAAGTGAAATTCTTATAGTC NGG 10 8 17 8 24 0 100 5 10
    240607040538_c9mx TTGTGGAAGGCAAGGATGTTGGAGAATAGGAG(+ATATAT)GTACCCAAGCCTCCTGCCTGTCTTAAGGTAG NGG 10 8 17 8 24 0 100 5 10
    240607040537_4lkc TAGAACTACCTTAAGACAGGCAGGAGGCTTGG(+ATATAT)GTACCTCCTATTCTCCAACATCCTTGCCTTC NGG 10 8 17 8 24 0 100 5 10