Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040450_fhqe AGCTACATCCTAAGTTCTGAGTGAGGGGGTAG(+ATATAT)GATCCACAGGAAGAAATAAGTCATGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240607040448_x9zu CTCCCCAAGCTCATGACTTATTTCTTCCTGTG(+ATATAT)GATCCTACCCCCTCACTCAGAACTTAGGATG NGG 10 8 17 8 24 0 100 5 10
    240607040447_oejx CCCAGCCCAGCCGCCACCAGCCTCTCTGATTG(+ATATAT)GATCCCCTTAACTGCATGCTCTTGAACCCCA NGG 10 8 17 8 24 0 100 5 10
    240607040445_npmt CCCCATGGGGTTCAAGAGCATGCAGTTAAGGG(+ATATAT)GATCCAATCAGAGAGGCTGGTGGCGGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607040444_f8pu GGCAAGTCTGCCTAGCTGTGCTCTGAGGACAG(+ATATAT)GATCCTAACTGACAGCCTACACTTTGTAGAT NGG 10 8 17 8 24 0 100 5 10
    240607040442_g0hp TGTTAATCTACAAAGTGTAGGCTGTCAGTTAG(+ATATAT)GATCCTGTCCTCAGAGCACAGCTAGGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240607040441_3oxp TCCTCAAAGCTAAAAACGGCTGCCTGTGTGTG(+ATATAT)GATCCTGCTCTCTCTTGAGGTCGTTACCTCA NGG 10 8 17 8 24 0 100 5 10
    240607040440_jp6s CAGTCTGAGGTAACGACCTCAAGAGAGAGCAG(+ATATAT)GATCCACACACAGGCAGCCGTTTTTAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240607040439_4e58 CATTTCCTCTGAGAACCGCTGAAGATTAGTAG(+ATATAT)GATCCCCACATCTCCCAGTTAAAGCCCAGTT NGG 10 8 17 8 24 0 100 5 10
    240607040437_j0x3 GGGAGAACTGGGCTTTAACTGGGAGATGTGGG(+ATATAT)GATCCTACTAATCTTCAGCGGTTCTCAGAGG NGG 10 8 17 8 24 0 100 5 10