Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040419_2sv7 GGAGGAGGCAGCCTTCAGGCGACAGGCGGGAG(+ATATAT)GTACCTTCAGTCAGGGGGCATAGGCAGAGGG NGG 10 8 17 8 24 0 100 5 10
    240607040418_yfzm GCCTGCCCTCTGCCTATGCCCCCTGACTGAAG(+ATATAT)GTACCTCCCGCCTGTCGCCTGAAGGCTGCCT NGG 10 8 17 8 24 0 100 5 10
    240607040416_ufj7 TGCCACCCCTTCCCTTATCAGGGACAGTATGG(+ATATAT)GTACCTCAGGTAACCCCAATTTCTGGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240607040414_rb8q AAGTCTCAGGCCAGAAATTGGGGTTACCTGAG(+ATATAT)GTACCCATACTGTCCCTGATAAGGGAAGGGG NGG 10 8 17 8 24 0 100 5 10
    240607040413_irre AGTCATCTCTACCACCAGTTCCAGAGAATGTG(+ATATAT)GTACCCTCTTCTGACCTCTGGGCACAAGGTA NGG 10 8 17 8 24 0 100 5 10
    240607040411_7ruf ATTCATACCTTGTGCCCAGAGGTCAGAAGAGG(+ATATAT)GTACCACATTCTCTGGAACTGGTGGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240607040410_62cs CTCTGAGCCAGCCCCCACAGCTGAGCTGCTCG(+ATATAT)GTACCTGACATGGGGTCTTCTGCTGCATTTT NGG 10 8 17 8 24 0 100 5 10
    240607040409_m1gv AAGCCAAAATGCAGCAGAAGACCCCATGTCAG(+ATATAT)GTACCGAGCAGCTCAGCTGTGGGGGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240607040407_tef8 CAGGACAACTCCTGCAGCACTGGGCTGTTGGG(+ATATAT)GATCCTGGGCACCCCTCTCATGGTGGCGGTG NGG 10 8 17 8 24 0 100 5 10
    240607040405_cb10 CCACCCACCGCCACCATGAGAGGGGTGCCCAG(+ATATAT)GATCCCCAACAGCCCAGTGCTGCAGGAGTTG NGG 10 8 17 8 24 0 100 5 10