Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040348_4k44 TGGCCTCCCAACCAAGGGAGCCATCATGGTGG(+ATATAT)GTACCAAGAAAGGCAAAGCACTTGGGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240607040347_no6g AAAAAAAAGCCCCAAGTGCTTTGCCTTTCTTG(+ATATAT)GTACCCACCATGATGGCTCCCTTGGTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240607040346_0zm8 TGTGGCCTCAGGCCTCAGTTCTTGGCCAACTG(+ATATAT)GTACCTGAATGTCTTGTTCTCATTTAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240607040345_m5my AAGCTGCCCCTAAATGAGAACAAGACATTCAG(+ATATAT)GTACCAGTTGGCCAAGAACTGAGGCCTGAGG NGG 10 8 17 8 24 0 100 5 10
    240607040343_ecu6 CAGTCACAGCGTGCCAGGGACTCAAGGAGAGG(+ATATAT)GTACCTGGAAGACTGTATGTCCTGAGGATAA NGG 10 8 17 8 24 0 100 5 10
    240607040341_ulmm AGCCCTTATCCTCAGGACATACAGTCTTCCAG(+ATATAT)GTACCCTCTCCTTGAGTCCCTGGCACGCTGT NGG 10 8 17 8 24 0 100 5 10
    240607040340_vs3x CAGTCACAGCGTGCCAGGGACTCAAGGAGAGG(+ATATAT)GTACCTGGAAGACTGTATGTCCTGAGGATAA NGG 10 8 17 8 24 0 100 5 10
    240607040338_id8d AGCCCTTATCCTCAGGACATACAGTCTTCCAG(+ATATAT)GTACCCTCTCCTTGAGTCCCTGGCACGCTGT NGG 10 8 17 8 24 0 100 5 10
    240607040336_742d CCTCCTTGGAGGATGTACTGTGATCATTCCTG(+ATATAT)GTACCTTAGGGCCAATCCCTAAGTGTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240607040334_wpgm TTAGGAAGCCACACTTAGGGATTGGCCCTAAG(+ATATAT)GTACCAGGAATGATCACAGTACATCCTCCAA NGG 10 8 17 8 24 0 100 5 10