Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040435_146c GGGCGCTCTCCATGGAACACATGCTCCGGCCG(+ATATAT)GATCCGCCATCCCTGAGGCTTACGACTTGCT NGG 10 8 17 8 24 0 100 5 10
    240607040433_ppok AAGAAAGCAAGTCGTAAGCCTCAGGGATGGCG(+ATATAT)GATCCGGCCGGAGCATGTGTTCCATGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240607040431_hgo9 CTAGTGTGACTGAGGGCCTAGGTTCAGCCCTG(+ATATAT)GTACCAACCCAAAAGGAAAAAAAAAAAATCA NGG 10 8 17 8 24 0 100 5 10
    240607040429_j0ya GCTCATGATTTTTTTTTTTTCCTTTTGGGTTG(+ATATAT)GTACCAGGGCTGAACCTAGGCCCTCAGTCAC NGG 10 8 17 8 24 0 100 5 10
    240607040428_f8ix AACCCTCCTCCCACGTCTCCATCAACCTCTGG(+ATATAT)GATCCAGAGGTAGCTTTGATGCCTGTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240607040426_rweg GGAAGCCCTCACAGGCATCAAAGCTACCTCTG(+ATATAT)GATCCCAGAGGTTGATGGAGACGTGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240607040425_cex7 GTTCGCCCAGGGGCTGGAGAGTTGGCTCAGTG(+ATATAT)GTACCACATGGTGGCTCACAACCATCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240607040424_elre TCTCATTACAGATGGTTGTGAGCCACCATGTG(+ATATAT)GTACCACTGAGCCAACTCTCCAGCCCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607040422_6vhk TGGACAAACAGCATGGCTATTTAACATTCTGG(+ATATAT)GATCCTGCAGAATCCTGCATCTTCCTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240607040421_12i8 TTCCCTACTTAGGAAGATGCAGGATTCTGCAG(+ATATAT)GATCCCAGAATGTTAAATAGCCATGCTGTTT NGG 10 8 17 8 24 0 100 5 10