Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041157_2d0t AGGAGACTAATGCCCTCTGATGATCTCTATGG(+ATATAT)GTACCAGGCACACATAGACATACAGGTATGC NGG 10 8 17 8 24 0 100 5 10
    240607041155_3sk0 TACCTGCATACCTGTATGTCTATGTGTGCCTG(+ATATAT)GTACCCATAGAGATCATCAGAGGGCATTAGT NGG 10 8 17 8 24 0 100 5 10
    240607041154_r51z AGTGGTTCAGAGAACTGGTTGTTCTTGCAGAG(+ATATAT)GATCCTAGGTTCAGTTCCTAGTACCTGCATG NGG 10 8 17 8 24 0 100 5 10
    240607041152_tml2 TCTGCCATGCAGGTACTAGGAACTGAACCTAG(+ATATAT)GATCCTCTGCAAGAACAACCAGTTCTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240607041150_ebo8 ACCCTCATACTGTCAGATACGTTTTGATAAGG(+ATATAT)GATCCAAGAGCAGTAAGGGTCAGTCTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240607041149_yw5x CTTTGTTGAAAGACTGACCCTTACTGCTCTTG(+ATATAT)GATCCCTTATCAAAACGTATCTGACAGTATG NGG 10 8 17 8 24 0 100 5 10
    240607041148_uksi CCAGGCATGTGCGTTAAGGGAGCTCTCAAACG(+ATATAT)GATCCCTCCAGCTGCATCCTGCCTTTCACCA NGG 10 8 17 8 24 0 100 5 10
    240607041146_5owf TTCTCTGGTGAAAGGCAGGATGCAGCTGGAGG(+ATATAT)GATCCGTTTGAGAGCTCCCTTAACGCACATG NGG 10 8 17 8 24 0 100 5 10
    240607041144_84u5 TCTGCTGTCTGCTGAGGGAGAGGGGACCTGTG(+ATATAT)GATCCTGACAACAGGCCACGCAGAGGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240607041142_sr10 CCGGCTCCCACCTCTGCGTGGCCTGTTGTCAG(+ATATAT)GATCCACAGGTCCCCTCTCCCTCAGCAGACA NGG 10 8 17 8 24 0 100 5 10