Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041236_zdqj ATCCTGCCCTCCCCAGCCAGCCCTCACAACTG(+ATATAT)GATCCAATGCTAATTTGTCAAAAAAGGCAAT NGG 10 8 17 8 24 0 100 5 10
    240607041235_eb1r AGACAATTGCCTTTTTTGACAAATTAGCATTG(+ATATAT)GATCCAGTTGTGAGGGCTGGCTGGGGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240607041234_y2fp CATTCCCTGCTCTCTGTTGACCTGTGTGGATG(+ATATAT)GTACCAGCAGACTTCCAACCCTCCAGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240607041233_kxpc TGACCAGAAGCTGGAGGGTTGGAAGTCTGCTG(+ATATAT)GTACCATCCACACAGGTCAACAGAGAGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607041232_js5v GTTTCTTGACTGCATATGAATAAGCATCTGAG(+ATATAT)GTACCAGAGAAAGGGGGTGCCTTTCCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240607041230_wnlb AGGCTCTCTGGGAAAGGCACCCCCTTTCTCTG(+ATATAT)GTACCTCAGATGCTTATTCATATGCAGTCAA NGG 10 8 17 8 24 0 100 5 10
    240607041229_qdtb GATCTCTGGCTTCCGTGACGCCGTCAATATTG(+ATATAT)GATCCTCACCAGGACCCCTCCTGGTTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240607041228_i8ii AACAGCAGGAAACCAGGAGGGGTCCTGGTGAG(+ATATAT)GATCCAATATTGACGGCGTCACGGAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240607041227_6iqx CTGGTGCCCACAGAGGTCACAGGAAAGCACAG(+ATATAT)GATCCTCTGGATGGCTGTGAACCACCATGTG NGG 10 8 17 8 24 0 100 5 10
    240607041226_13px ATACCCACATGGTGGTTCACAGCCATCCAGAG(+ATATAT)GATCCTGTGCTTTCCTGTGACCTCTGTGGGC NGG 10 8 17 8 24 0 100 5 10