Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041449_88zy ATGGAACTTTGGCCTCCTTTACGCTGGGAAAG(+ATATAT)GATCCCACCACTGAGCTACAACCCAGCCCTT NGG 10 8 17 8 24 0 100 5 10
    240607041448_dm3l GCTAGAAGGGCTGGGTTGTAGCTCAGTGGTGG(+ATATAT)GATCCTTTCCCAGCGTAAAGGAGGCCAAAGT NGG 10 8 17 8 24 0 100 5 10
    240607041446_alcw CTTGTGGGTGTCTGGAGAATCCCTGGGCCAGG(+ATATAT)GATCCCAGTGCTGCAGTGGGCCAGAAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240607041444_884s GGGGGGAAGCTTCTGGCCCACTGCAGCACTGG(+ATATAT)GATCCCTGGCCCAGGGATTCTCCAGACACCC NGG 10 8 17 8 24 0 100 5 10
    240607041443_q3xp TTCCCCATTGGGAAGCATAAAGCACTTTTGTG(+ATATAT)GTACCTTTCACTGGTAAATATTTATATCACA NGG 10 8 17 8 24 0 100 5 10
    240607041441_s67z TTCAATGTGATATAAATATTTACCAGTGAAAG(+ATATAT)GTACCACAAAAGTGCTTTATGCTTCCCAATG NGG 10 8 17 8 24 0 100 5 10
    240607041440_zst3 TAAGACTGCTAGGGGATGGAAAGATCTGGGTG(+ATATAT)GATCCATGTTTTCTCTGTTTGAAATACCTTT NGG 10 8 17 8 24 0 100 5 10
    240607041438_48nn TTTTAAAAGGTATTTCAAACAGAGAAAACATG(+ATATAT)GATCCACCCAGATCTTTCCATCCCCTAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607041437_rhxm TTGAAGAAAATGTGTACTGTCTCAAAAGAAGG(+ATATAT)GATCCCTCTTACAGTGCAGAGGATGTTGGAC NGG 10 8 17 8 24 0 100 5 10
    240607041436_exrs CGGCTGTCCAACATCCTCTGCACTGTAAGAGG(+ATATAT)GATCCCTTCTTTTGAGACAGTACACATTTTC NGG 10 8 17 8 24 0 100 5 10