Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041434_6l4b GGTCCCGTCCTGGCAGGGGTTGAAGGGGAGAG(+ATATAT)GTACCCCCACATGCTGGGGACCTCAGCACAG NGG 10 8 17 8 24 0 100 5 10
    240607041432_8pgx TGAAACTGTGCTGAGGTCCCCAGCATGTGGGG(+ATATAT)GTACCTCTCCCCTTCAACCCCTGCCAGGACG NGG 10 8 17 8 24 0 100 5 10
    240607041431_qbc6 AATGAAAGTGTGTTCTACTGTCAGTATAACTG(+ATATAT)GATCCTCAGACCGGCACAGTGGGCTCCGGGA NGG 10 8 17 8 24 0 100 5 10
    240607041430_7di7 GAGTATCCCGGAGCCCACTGTGCCGGTCTGAG(+ATATAT)GATCCAGTTATACTGACAGTAGAACACACTT NGG 10 8 17 8 24 0 100 5 10
    240607041428_9m5i ACGTGTCAGGTAGAGCTTTCTAAGTGAGCTGG(+ATATAT)GATCCTGACTCACGTGTCATAGACTTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240607041427_lgix CTGCTCTTACAAGTCTATGACACGTGAGTCAG(+ATATAT)GATCCCAGCTCACTTAGAAAGCTCTACCTGA NGG 10 8 17 8 24 0 100 5 10
    240607041425_pagt CCAGGCAGGCTGGTCCCTGCTGTGATTAATGG(+ATATAT)GTACCCCAAAGTACAAACTAACGACAATGGA NGG 10 8 17 8 24 0 100 5 10
    240607041423_2xvy CAGCATCCATTGTCGTTAGTTTGTACTTTGGG(+ATATAT)GTACCCATTAATCACAGCAGGGACCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240607041422_glow GAGCGCAGTGGGATGAACTCACAGCAGTACTG(+ATATAT)GTACCATTTTAGATTTCTTTCTTGCACTATA NGG 10 8 17 8 24 0 100 5 10
    240607041421_99ns TGAAGTATAGTGCAAGAAAGAAATCTAAAATG(+ATATAT)GTACCAGTACTGCTGTGAGTTCATCCCACTG NGG 10 8 17 8 24 0 100 5 10