Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041701_0z4u TGAGAGAAGGGCTGGGAGCCAGCCAAGGGATG(+ATATAT)GTACCTTTGTTGTGACGGGGCTACACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240607041700_c4wk GCTGAGAAGGGTGTAGCCCCGTCACAACAAAG(+ATATAT)GTACCATCCCTTGGCTGGCTCCCAGCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240607041659_9ccn AGAAGTCCAGATACTCCATTCTTGAGTGTCAG(+ATATAT)GATCCCAGGCAAAACTGGCAATACAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240607041656_ixh4 GTTTCCTGGATTGTATTGCCAGTTTTGCCTGG(+ATATAT)GATCCTGACACTCAAGAATGGAGTATCTGGA NGG 10 8 17 8 24 0 100 5 10
    240607041655_eh8w GTGCTTGCTGCCAGGTTTGACCACGTGAGTTG(+ATATAT)GATCCCTGGAACCCACATGGTGGAAAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240607041653_mnr9 CAATTCTCTCTTTCCACCATGTGGGTTCCAGG(+ATATAT)GATCCAACTCACGTGGTCAAACCTGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240607041652_gx1o GTGGTCATTGAGTGCCACCAGTACATCTGATG(+ATATAT)GATCCAGTTGGCACGTCATGACAGATTGACT NGG 10 8 17 8 24 0 100 5 10
    240607041651_igjb ACACAAGTCAATCTGTCATGACGTGCCAACTG(+ATATAT)GATCCATCAGATGTACTGGTGGCACTCAATG NGG 10 8 17 8 24 0 100 5 10
    240607041649_e5w9 AGGACGGAGCCTAGGACTCTGTACCTGCCAAG(+ATATAT)GATCCCAGAGCCCAAGTATGTGGGGCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240607041648_i12v CCAAACCAAAGCCCCACATACTTGGGCTCTGG(+ATATAT)GATCCTTGGCAGGTACAGAGTCCTAGGCTCC NGG 10 8 17 8 24 0 100 5 10