Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041616_3fbz TCTTACTAAAGTAATGGGCTCGTTTGCTATGG(+ATATAT)GATCCAGAACAACACTGTTTAGTATGTCCTT NGG 10 8 17 8 24 0 100 5 10
    240607041615_w79x TTTGGAAGGACATACTAAACAGTGTTGTTCTG(+ATATAT)GATCCCATAGCAAACGAGCCCATTACTTTAG NGG 10 8 17 8 24 0 100 5 10
    240607041613_n9io TCGTTACCTCTAGAGGAAAGGCTGCTCGCCTG(+ATATAT)GTACCAAGACTACAGACAGGTGACAGAGCAA NGG 10 8 17 8 24 0 100 5 10
    240607041612_9xjx TCTGATTGCTCTGTCACCTGTCTGTAGTCTTG(+ATATAT)GTACCAGGCGAGCAGCCTTTCCTCTAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240607041610_wabe GTCTATGACCCCATCCTTTAGGTCAGTTCTGG(+ATATAT)GATCCAATCTCTGGTCTTCACACTTTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240607041609_8yfy TGCCTGCTGCAAAGTGTGAAGACCAGAGATTG(+ATATAT)GATCCCAGAACTGACCTAAAGGATGGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240607041608_x4zs GTGCTCACCTGCATCAAACACAAAACACGATG(+ATATAT)GTACCTACTCTTTTCCCGAGTCCATGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240607041607_smlq CTCCCGTAGCCATGGACTCGGGAAAAGAGTAG(+ATATAT)GTACCATCGTGTTTTGTGTTTGATGCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240607041605_03ii ATTTGGATTTAAATGGATTTGAAAGTCTTTGG(+ATATAT)GATCCTTGAAGGATTTGTAATAATCACTTTC NGG 10 8 17 8 24 0 100 5 10
    240607041604_d199 TTAGAGAAAGTGATTATTACAAATCCTTCAAG(+ATATAT)GATCCCAAAGACTTTCAAATCCATTTAAATC NGG 10 8 17 8 24 0 100 5 10