Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041717_xsk9 GCTCGCTGTGAAAACACAAGGACCTGAGCTGG(+ATATAT)GATCCTTGGTACCCAGAGAAAAAAATCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607041716_doq6 CCAGACCCAGATTTTTTTCTCTGGGTACCAAG(+ATATAT)GATCCCAGCTCAGGTCCTTGTGTTTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240607041713_4sm6 TAGCAAACCAAAAAACACCCCTTTTCCCAAGG(+ATATAT)GATCCTACATCTCTAGCATTTGTGAAACCTG NGG 10 8 17 8 24 0 100 5 10
    240607041712_wnay GACCCCAGGTTTCACAAATGCTAGAGATGTAG(+ATATAT)GATCCCTTGGGAAAAGGGGTGTTTTTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240607041710_7bw3 ACTCCCCCATAGGGTCAAACGGCTCAACCAAG(+ATATAT)GTACCTGGCCAGGACACAGTCCTCATGCTAG NGG 10 8 17 8 24 0 100 5 10
    240607041708_0o1y AGGAGCTAGCATGAGGACTGTGTCCTGGCCAG(+ATATAT)GTACCTTGGTTGAGCCGTTTGACCCTATGGG NGG 10 8 17 8 24 0 100 5 10
    240607041707_5y9n CGGTCTGGAGCCAGCGGAGAAGGAGGAGATTG(+ATATAT)GATCCCTCAGAAGGGCTCCCCGTGCAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240607041706_haaf GGGGTGGGGCTGCACGGGGAGCCCTTCTGAGG(+ATATAT)GATCCAATCTCCTCCTTCTCCGCTGGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240607041704_urca AGATTTTGAGATCACATCGTTCTCCCCTCAAG(+ATATAT)GATCCCCTGATTAGTGGGAGACTAAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240607041703_8qb1 GGGGGCAGGTCTTAGTCTCCCACTAATCAGGG(+ATATAT)GATCCTTGAGGGGAGAACGATGTGATCTCAA NGG 10 8 17 8 24 0 100 5 10