Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607041953_0nke TACCCCTCCTGTGTGTGAAGGCTCTGCTGAAG(+ATATAT)GTACCTTTGGGTCTGGTCTGACCTTACTGCT NGG 10 8 17 8 24 0 100 5 10
    240607041952_il43 GACAAAGCAGTAAGGTCAGACCAGACCCAAAG(+ATATAT)GTACCTTCAGCAGAGCCTTCACACACAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607041950_fogz CGGGCCACCACCTGCCTCTCTCTTATCCAGTG(+ATATAT)GTACCGCCCAGGCCTTAAATCACCCCGTTCC NGG 10 8 17 8 24 0 100 5 10
    240607041948_xjzo CACTGGGAACGGGGTGATTTAAGGCCTGGGCG(+ATATAT)GTACCACTGGATAAGAGAGAGGCAGGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240607041946_dbda GGTCACAGGGACAGCTCCGCTGCTGCCTCTGG(+ATATAT)GTACCTTGAAAAGATGCCCACCACGCACCTT NGG 10 8 17 8 24 0 100 5 10
    240607041945_xxhn AAAGAAAGGTGCGTGGTGGGCATCTTTTCAAG(+ATATAT)GTACCCAGAGGCAGCAGCGGAGCTGTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240607041943_ps8u CTGCTGCCCCTGGGTGCAGGCAGTAGCCAGGG(+ATATAT)GATCCAGGGCCTCAGATCCAGGGTCCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240607041940_7z8i GTCCGATGCAGGACCCTGGATCTGAGGCCCTG(+ATATAT)GATCCCCTGGCTACTGCCTGCACCCAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240607041939_5f9x CTGCCGGGGAGATGGGCAGGGTGCACTCTGAG(+ATATAT)GTACCCGCTCCTACAGTCCCAGAACACCCCT NGG 10 8 17 8 24 0 100 5 10
    240607041937_9660 AAGAGAGGGGTGTTCTGGGACTGTAGGAGCGG(+ATATAT)GTACCTCAGAGTGCACCCTGCCCATCTCCCC NGG 10 8 17 8 24 0 100 5 10