Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040234_m94c CAACCCATAGGGTACATATTTTGTCACAAAAG(+ATATAT)GTACCAGATGCATGCCAAACCAAACAACCTG NGG 10 8 17 8 24 0 100 5 10
    240607040233_m1os TAATCCAGGTTGTTTGGTTTGGCATGCATCTG(+ATATAT)GTACCTTTTGTGACAAAATATGTACCCTATG NGG 10 8 17 8 24 0 100 5 10
    240607040231_7xwm TGTACAGCAGCCATCTCTTGAGGTCACACCTG(+ATATAT)GTACCTACATAGGCAAGAAGGCTTCGGGATA NGG 10 8 17 8 24 0 100 5 10
    240607040230_36ko CTATGTATCCCGAAGCCTTCTTGCCTATGTAG(+ATATAT)GTACCAGGTGTGACCTCAAGAGATGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240607040229_r4bt TTCCCTGAGACTTGGTACTCTATGAGGTGGAG(+ATATAT)GTACCCATCCCTGATCTGAACCCTTTTCTGG NGG 10 8 17 8 24 0 100 5 10
    240607040227_1v7i CTAACCCAGAAAAGGGTTCAGATCAGGGATGG(+ATATAT)GTACCTCCACCTCATAGAGTACCAAGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240607040225_jx3t CAGACGCAGACGGGGCAGCAAGGACGGCCCTG(+ATATAT)GATCCAACTCTTCAGCTACTGCTCCCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240607040223_3xiy GGCTTGGCTTGGGAGCAGTAGCTGAAGAGTTG(+ATATAT)GATCCAGGGCCGTCCTTGCTGCCCCGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240607040222_a1s4 TCCCCTTCATTCCATGATGAGGACACTGCCAG(+ATATAT)GATCCAGGGGTAAAGCACTGAGCTCAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607040219_dlpf GAGCTCTGCCTGAGCTCAGTGCTTTACCCCTG(+ATATAT)GATCCTGGCAGTGTCCTCATCATGGAATGAA NGG 10 8 17 8 24 0 100 5 10