Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040201_gusv AATTAAATAACTTTCATGGGGTTCAGGGTTAG(+ATATAT)GATCCCCAGCCTTCCCTCTTGGGGGAAACCG NGG 10 8 17 8 24 0 100 5 10
    240607040159_rmuj TGCCTCGGTTTCCCCCAAGAGGGAAGGCTGGG(+ATATAT)GATCCTAACCCTGAACCCCATGAAAGTTATT NGG 10 8 17 8 24 0 100 5 10
    240607040157_tlqb ATCCCAGTTTGGGATACGTTCTCTCGCACCAG(+ATATAT)GTACCCATCCATGGTGCCTTTGTTTCTTCGG NGG 10 8 17 8 24 0 100 5 10
    240607040156_dywf ACAGTCCGAAGAAACAAAGGCACCATGGATGG(+ATATAT)GTACCTGGTGCGAGAGAACGTATCCCAAACT NGG 10 8 17 8 24 0 100 5 10
    240607040154_awp2 AGTGCCACCCTCCCAGCTGGCCCTGTGGTTGG(+ATATAT)GATCCTGCCTGACTTCTTCCAGCTATCCCCT NGG 10 8 17 8 24 0 100 5 10
    240607040153_q02t CCCCAAGGGGATAGCTGGAAGAAGTCAGGCAG(+ATATAT)GATCCCAACCACAGGGCCAGCTGGGAGGGTG NGG 10 8 17 8 24 0 100 5 10
    240607040150_0yqx AAAGCTGACATAGCACATTCTTGGCACTCCGG(+ATATAT)GTACCAAGGAGGCTATTGGGGGCGGAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240607040149_2tv4 TTGCATGCTCTCCGCCCCCAATAGCCTCCTTG(+ATATAT)GTACCCGGAGTGCCAAGAATGTGCTATGTCA NGG 10 8 17 8 24 0 100 5 10
    240607040147_l7l2 AAAGTCCACAAAGTTTATTGAGCCCTGTCTAG(+ATATAT)GATCCAGAGGGAACTGGGAGTCTGTGCCAGA NGG 10 8 17 8 24 0 100 5 10
    240607040146_psv9 CAGAATCTGGCACAGACTCCCAGTTCCCTCTG(+ATATAT)GATCCTAGACAGGGCTCAATAAACTTTGTGG NGG 10 8 17 8 24 0 100 5 10