Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607040145_clzf AGGCTACTTGTAAGTCTCAGGGGGGAATATTG(+ATATAT)GATCCTGTCACATAGAAGCCTCCCCTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607040143_fbn4 CTGTTGCTGGAGGGGAGGCTTCTATGTGACAG(+ATATAT)GATCCAATATTCCCCCCTGAGACTTACAAGT NGG 10 8 17 8 24 0 100 5 10
    240607040142_k3zt CCTCACTCTCCTCTAAACGGTTCTAGTCCTAG(+ATATAT)GATCCAAGGGACACTACGGGTCTCCCCATCA NGG 10 8 17 8 24 0 100 5 10
    240607040139_plmq AGAGTTGATGGGGAGACCCGTAGTGTCCCTTG(+ATATAT)GATCCTAGGACTAGAACCGTTTAGAGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240607040137_9i4h CCAAGGTAGCCCTCTGTTCTTGAGACCCTCGG(+ATATAT)GTACCAAGCTACATGCCCCCTAGCAGAACTC NGG 10 8 17 8 24 0 100 5 10
    240607040136_5ek0 AAATAGAGTTCTGCTAGGGGGCATGTAGCTTG(+ATATAT)GTACCCGAGGGTCTCAAGAACAGAGGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240607040134_qrgi ACCCACTATACAGCTCTATTCCTAACCAGTGG(+ATATAT)GATCCCCAAATCCAGGCCCTCAGCAGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240607040132_nt15 CACGCCCCCACTGCTGAGGGCCTGGATTTGGG(+ATATAT)GATCCCACTGGTTAGGAATAGAGCTGTATAG NGG 10 8 17 8 24 0 100 5 10
    240607040130_pdk0 GCAGTCCTCCCTGTTACAGGGGCGACAGGTAG(+ATATAT)GTACCACCTGGTTATGCCAATCTACTCATTT NGG 10 8 17 8 24 0 100 5 10
    240607040129_tga7 TTCTAAAATGAGTAGATTGGCATAACCAGGTG(+ATATAT)GTACCTACCTGTCGCCCCTGTAACAGGGAGG NGG 10 8 17 8 24 0 100 5 10