Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034756_iz3n GAAGTCACTCACTGTAGGTGTCTGCCTCTGGG(+ATATAT)GATCCTTGCAGTGTCCTTTCACAGTGACCCT NGG 10 8 17 8 24 0 100 5 10
    240607034755_g8di GGATGGGGAGATGTGCAGGGTGAGGGCAATTG(+ATATAT)GTACCACAATGCCAAGGTCATGAAGCTAGGA NGG 10 8 17 8 24 0 100 5 10
    240607034754_z60d CTCTGTCCTAGCTTCATGACCTTGGCATTGTG(+ATATAT)GTACCAATTGCCCTCACCCTGCACATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240607034753_r29c ACATGGCTGTCTCTCAGCAGCCATGACTGTAG(+ATATAT)GTACCTAGGTTTCATACAGCTCCATGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240607034751_eplg CTTATGCCTACATGGAGCTGTATGAAACCTAG(+ATATAT)GTACCTACAGTCATGGCTGCTGAGAGACAGC NGG 10 8 17 8 24 0 100 5 10
    240607034749_gr5a ACTGAGACCTGGACCTGATGCTTGAAAGGCGG(+ATATAT)GTACCTTATTGACTGAGCTATGTCAACAGTT NGG 10 8 17 8 24 0 100 5 10
    240607034748_orfr TCTTGAACTGTTGACATAGCTCAGTCAATAAG(+ATATAT)GTACCCGCCTTTCAAGCATCAGGTCCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240607034747_4y5a GAATTCAGTTAGCAACACTACAGGATATCCTG(+ATATAT)GTACCAATGAATCTGATAATTCTAAATTAAA NGG 10 8 17 8 24 0 100 5 10
    240607034745_h95o CAAGTTTTAATTTAGAATTATCAGATTCATTG(+ATATAT)GTACCAGGATATCCTGTAGTGTTGCTAACTG NGG 10 8 17 8 24 0 100 5 10
    240607034744_dzh2 CTCACCCCTTACTGAGAATCTATTCACAATTG(+ATATAT)GTACCTATTGGGAAAGGGAAAACCAGCTTTT NGG 10 8 17 8 24 0 100 5 10