Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034842_ei55 CCAAATGCAGAGAAAGGAGCTTAGGTCCAAAG(+ATATAT)GTACCCAGCTATTTGTTTTAAAGAAATAGGA NGG 10 8 17 8 24 0 100 5 10
    240607034840_oo9r TAGTCTAGCAATGTGGGGGTCAGGGATGGGAG(+ATATAT)GATCCATCGCTGGCTTGCTGCTTGCTAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607034839_r43r GAGCTCTGCTAGCAAGCAGCAAGCCAGCGATG(+ATATAT)GATCCTCCCATCCCTGACCCCCACATTGCTA NGG 10 8 17 8 24 0 100 5 10
    240607034838_q0n6 GCAATGTAACAGATTCTGGTTTAAATATCTGG(+ATATAT)GATCCACATATGATCTCTGAGCTCCTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240607034836_xxlz AGCCCTTTGGAGGAGCTCAGAGATCATATGTG(+ATATAT)GATCCCAGATATTTAAACCAGAATCTGTTAC NGG 10 8 17 8 24 0 100 5 10
    240607034835_s2ga GGTGTCAGTGTTTGGAAGCTGATTATGGGATG(+ATATAT)GATCCCTGCATATGGCAGTCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607034833_jx7m GGATGGACCATCTAGTGACTGCCATATGCAGG(+ATATAT)GATCCATCCCATAATCAGCTTCCAAACACTG NGG 10 8 17 8 24 0 100 5 10
    240607034831_59wh GGATGGACCATGTAGAGACTGCCATATCCAGG(+ATATAT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240607034829_5h7o GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+ATATAT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607034828_phnh AGTGATGACTCAGATGACGACACTTTATAATG(+ATATAT)GTACCTGGTTGGTGGACAACTCACACAAACT NGG 10 8 17 8 24 0 100 5 10