Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034912_b41h ACAAACCATGGCTAATTGTAACCCCTACCTGG(+ATATAT)GATCCATCTCCAATGATATTCCAGTCATTGT NGG 10 8 17 8 24 0 100 5 10
    240607034910_7sd8 CACAGCACAAACTACCAAATTTTGATTTTTTG(+ATATAT)GATCCCAGCAATAATTTTTAATAGTTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240607034909_gyxc CCAGTTTGAAAACTATTAAAAATTATTGCTGG(+ATATAT)GATCCAAAAAATCAAAATTTGGTAGTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240607034908_7x4f ACCCATCTCAGGTTATAGTCCACATAAGCGTG(+ATATAT)GATCCTACATGTTTCTTGATTAGGATAGTCT NGG 10 8 17 8 24 0 100 5 10
    240607034907_6x5s TAGGTAGACTATCCTAATCAAGAAACATGTAG(+ATATAT)GATCCACGCTTATGTGGACTATAACCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240607034905_51g2 TGCCTGTACCTTCAAATGTAAAGTAAGCCACG(+ATATAT)GTACCTTGTGAGACATGTGTTCCTTGTGTTC NGG 10 8 17 8 24 0 100 5 10
    240607034903_506b CCAAGGAACACAAGGAACACATGTCTCACAAG(+ATATAT)GTACCGTGGCTTACTTTACATTTGAAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240607034902_h051 AGGTGACGGTCTGCTCATTTGTCAATGGGATG(+ATATAT)GTACCTTCCTTTCTCTTCATGGATTGCATCC NGG 10 8 17 8 24 0 100 5 10
    240607034901_1dis TGCAGGGATGCAATCCATGAAGAGAAAGGAAG(+ATATAT)GTACCATCCCATTGACAAATGAGCAGACCGT NGG 10 8 17 8 24 0 100 5 10
    240607034859_ejpu ATGTTCTTTGTACCCACGCAGGGTTCTGTCTG(+ATATAT)GTACCCCACATGTCAGCTTAAAGGACTTCTT NGG 10 8 17 8 24 0 100 5 10