Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035011_9f0p GTTGTCTTCAGGCACACCAGAAGTGGGCATTG(+ATATAT)GATCCCGTTACGGATGATTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607035009_iqhg ACCACATGGTGGCTCACAATCATCCGTAACGG(+ATATAT)GATCCAATGCCCACTTCTGGTGTGCCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607035008_zt4d TTTAATCTACCAAATTACATGATTTGAGAGTG(+ATATAT)GTACCCAAGGAGAGAATTCAGTTCTGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240607035006_j2y2 ACAGCAACAACAGAACTGAATTCTCTCCTTGG(+ATATAT)GTACCACTCTCAAATCATGTAATTTGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240607035004_pohb AGATCAAGTCCCTTCTCACCCTGTTAAGATTG(+ATATAT)GTACCATATACTGGCAATGAACATACACTGG NGG 10 8 17 8 24 0 100 5 10
    240607035003_pvss AGAAACCAGTGTATGTTCATTGCCAGTATATG(+ATATAT)GTACCAATCTTAACAGGGTGAGAAGGGACTT NGG 10 8 17 8 24 0 100 5 10
    240607035002_25h3 TCTTAAATTTTTCTTTAGAAATGCTTCAGTTG(+ATATAT)GATCCAAACATTTTTCCAACTATTAAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240607035001_p3nh GTGATTTTTCTTAATAGTTGGAAAAATGTTTG(+ATATAT)GATCCAACTGAAGCATTTCTAAAGAAAAATT NGG 10 8 17 8 24 0 100 5 10
    240607034959_26y8 AAAAATCACCTGTGGAAGCACAAAATTCCATG(+ATATAT)GATCCAGAGTGGAGAGTGTTCTGTTCTATGC NGG 10 8 17 8 24 0 100 5 10
    240607034958_ubn9 TTAATGCATAGAACAGAACACTCTCCACTCTG(+ATATAT)GATCCATGGAATTTTGTGCTTCCACAGGTGA NGG 10 8 17 8 24 0 100 5 10