Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035058_6kys GGCTGATACACTGAGTGCAAGAGTACTGGAGG(+ATATAT)GATCCAGTGAGTCAAACCAGTAAGCAGTGAG NGG 10 8 17 8 24 0 100 5 10
    240607035057_hafp CACACCTCACTGCTTACTGGTTTGACTCACTG(+ATATAT)GATCCCTCCAGTACTCTTGCACTCAGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240607035055_wh7v CATTATCTTTGACAATTTAAACAAGATCAAAG(+ATATAT)GTACCCAAAGGGTTCTGGGAGCTAAGATCTC NGG 10 8 17 8 24 0 100 5 10
    240607035054_nlpw CTATGGAGATCTTAGCTCCCAGAACCCTTTGG(+ATATAT)GTACCTTTGATCTTGTTTAAATTGTCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607035053_3pca CATTATCTTTGACAATTTAAACAAGATCAAAG(+ATATAT)GTACCCAAAGGGTTCTGGGAGCTAAGATCTC NGG 10 8 17 8 24 0 100 5 10
    240607035051_tu48 CTATGGAGATCTTAGCTCCCAGAACCCTTTGG(+ATATAT)GTACCTTTGATCTTGTTTAAATTGTCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607035050_pbqb GTTTTCTGAAGTCATGGATTAAGTGATCTGCG(+ATATAT)GATCCACCCAGGCACAGTCAGATCAAAGCGT NGG 10 8 17 8 24 0 100 5 10
    240607035048_uf3k TTGCAACGCTTTGATCTGACTGTGCCTGGGTG(+ATATAT)GATCCGCAGATCACTTAATCCATGACTTCAG NGG 10 8 17 8 24 0 100 5 10
    240607035046_c0wa GGGAGTCTGAGTTCCTCTCCTGATGTCTAGGG(+ATATAT)GTACCAGGTTCGCATGTAGTACAAACACATG NGG 10 8 17 8 24 0 100 5 10
    240607035044_n0ta ACCTCCATGTGTTTGTACTACATGCGAACCTG(+ATATAT)GTACCCCTAGACATCAGGAGAGGAACTCAGA NGG 10 8 17 8 24 0 100 5 10