Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035140_vgxy GTATGGGTTAGGAAGCGCGGCTGTAGACAATG(+ATATAT)GTACCTGTGACACACTGAGGGGCTACGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607035139_z9ob TAATCCTGCCGTAGCCCCTCAGTGTGTCACAG(+ATATAT)GTACCATTGTCTACAGCCGCGCTTCCTAACC NGG 10 8 17 8 24 0 100 5 10
    240607035138_vdmd AGACAGTAACACTTGGAAGGGACAGCAGTGTG(+ATATAT)GATCCAGAATGCCAGAATGCAAGTCCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240607035137_0wnp CCTGACAGCAGGACTTGCATTCTGGCATTCTG(+ATATAT)GATCCACACTGCTGTCCCTTCCAAGTGTTAC NGG 10 8 17 8 24 0 100 5 10
    240607035136_iu54 CCATGATATCCCTCTCTCTGAGTCACGCGTTG(+ATATAT)GATCCTAGAATTATTCCTGCGGCTTCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240607035134_j9se AGGAGGGGGAGAAGCCGCAGGAATAATTCTAG(+ATATAT)GATCCAACGCGTGACTCAGAGAGAGGGATAT NGG 10 8 17 8 24 0 100 5 10
    240607035133_30wk CCCGCCTGCAGCGCGGCGGGTAGGCGGTTTGG(+ATATAT)GATCCTCGCCCAACTCCGCTTACTTTAATGA NGG 10 8 17 8 24 0 100 5 10
    240607035132_gf3q CGTTCTCATTAAAGTAAGCGGAGTTGGGCGAG(+ATATAT)GATCCCAAACCGCCTACCCGCCGCGCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240607035129_ea5c TTTTTGATGTTGTTCTTTTGTCAAGCCCCCAG(+ATATAT)GTACCAAAAAACAGCGTCCCCAGTCGGGTAA NGG 10 8 17 8 24 0 100 5 10
    240607035128_zsdk GTTTCTTACCCGACTGGGGACGCTGTTTTTTG(+ATATAT)GTACCTGGGGGCTTGACAAAAGAACAACATC NGG 10 8 17 8 24 0 100 5 10