Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035126_8a7b CAGTACATCTCTTAGGTGACAGAGGCCAATGG(+ATATAT)GATCCACACCATTGGCCTCTCCTCTGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240607035125_w8p1 ACATTAGCACCAGAGGAGAGGCCAATGGTGTG(+ATATAT)GATCCCATTGGCCTCTGTCACCTAAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240607035123_dzy0 GTTTGGCTGTTTGTTTCTGTTTTGTCAGGGTG(+ATATAT)GATCCAAGAGAATGGGGCATTAGTCAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240607035122_1ihl TGCACATGACTGACTAATGCCCCATTCTCTTG(+ATATAT)GATCCACCCTGACAAAACAGAAACAAACAGC NGG 10 8 17 8 24 0 100 5 10
    240607035120_gkla CTCAGGTGGGTAACCTGAGTTCAGAGTCCAAG(+ATATAT)GTACCAGTAGCAACGCAGGCAAACTAGTTGT NGG 10 8 17 8 24 0 100 5 10
    240607035119_koqp GATGTACAACTAGTTTGCCTGCGTTGCTACTG(+ATATAT)GTACCTTGGACTCTGAACTCAGGTTACCCAC NGG 10 8 17 8 24 0 100 5 10
    240607035117_o95b AGACACACAGAGCGGAGGGGAGGGACAACCTG(+ATATAT)GTACCAGTCTGGAAATCTGATTGGCCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240607035116_8mbu CCATCTGCTGGGCCAATCAGATTTCCAGACTG(+ATATAT)GTACCAGGTTGTCCCTCCCCTCCGCTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240607035115_maho TTTTCCCTGAGTGCCAACCTTAAAGATGGGTG(+ATATAT)GTACCAACTTCACTTGGCTAGTAGAGCTAGC NGG 10 8 17 8 24 0 100 5 10
    240607035114_bqlm CCTCAGCTAGCTCTACTAGCCAAGTGAAGTTG(+ATATAT)GTACCACCCATCTTTAAGGTTGGCACTCAGG NGG 10 8 17 8 24 0 100 5 10