Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035154_ejh5 AGCCTTCTCCGGCTGCCTCCTGAAGACACAAG(+ATATAT)GATCCTTCGCACCATGGTACTTTGAAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240607035153_9neu CAGGTGAGCTTTCAAAGTACCATGGTGCGAAG(+ATATAT)GATCCTTGTGTCTTCAGGAGGCAGCCGGAGA NGG 10 8 17 8 24 0 100 5 10
    240607035152_23c9 TTACAGACACGAAGTCATTATACACATTAGTG(+ATATAT)GATCCTTGGGACCACAGGGACCTCGTGCAAT NGG 10 8 17 8 24 0 100 5 10
    240607035150_go2t AGGACATTGCACGAGGTCCCTGTGGTCCCAAG(+ATATAT)GATCCACTAATGTGTATAATGACTTCGTGTC NGG 10 8 17 8 24 0 100 5 10
    240607035148_880t GACAGAACCTTGCAGATAATGAGCTTTTGGAG(+ATATAT)GATCCTCCAAGCTCACCCAGAATCGGAACGG NGG 10 8 17 8 24 0 100 5 10
    240607035147_c4f4 TGATTCCGTTCCGATTCTGGGTGAGCTTGGAG(+ATATAT)GATCCTCCAAAAGCTCATTATCTGCAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240607035146_wa07 ACAGTTTAATTCACTGAGGAAAAAAGGATTTG(+ATATAT)GTACCTAAAATGGCCAAATAATGATGTATTT NGG 10 8 17 8 24 0 100 5 10
    240607035144_nyz2 ACTTTAAATACATCATTATTTGGCCATTTTAG(+ATATAT)GTACCAAATCCTTTTTTCCTCAGTGAATTAA NGG 10 8 17 8 24 0 100 5 10
    240607035142_rj1n GGCTCACAACTGCCTGTAACTCCAGCTCCAGG(+ATATAT)GATCCAGTGTGCACACACACATGTGCACACA NGG 10 8 17 8 24 0 100 5 10
    240607035141_495x GCGTATGTGTGCACATGTGTGTGTGCACACTG(+ATATAT)GATCCCTGGAGCTGGAGTTACAGGCAGTTGT NGG 10 8 17 8 24 0 100 5 10