Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035209_qfvh CTCCTGTGGCCTCTCCAGCAGGAGACCCACAG(+ATATAT)GATCCCATGCAATGGCTGCTTTGCTCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607035207_hgn5 GCCTACTGAGGAGCAAAGCAGCCATTGCATGG(+ATATAT)GATCCTGTGGGTCTCCTGCTGGAGAGGCCAC NGG 10 8 17 8 24 0 100 5 10
    240607035206_p8eh GCTAAATGGATGCTTCAGTCCATGGCTGAGAG(+ATATAT)GTACCGCCTGCACCAGCAAAGTGGAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240607035205_f32l AGCTTCAGCACTCCACTTTGCTGGTGCAGGCG(+ATATAT)GTACCTCTCAGCCATGGACTGAAGCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240607035203_ntk5 TAGCTTTGTTACATCCTAGCAAGATATTCCTG(+ATATAT)GATCCCTGCTGCGCATTCTGATGTGAATACC NGG 10 8 17 8 24 0 100 5 10
    240607035201_hhr2 ACCTTGGTATTCACATCAGAATGCGCAGCAGG(+ATATAT)GATCCAGGAATATCTTGCTAGGATGTAACAA NGG 10 8 17 8 24 0 100 5 10
    240607035200_iodp ATATTTTTTCACCTAATCTCGGGGTCTTACTG(+ATATAT)GTACCCTCACCCAAGTGCCTATGAAGCACGA NGG 10 8 17 8 24 0 100 5 10
    240607035158_opqp GAACCTCGTGCTTCATAGGCACTTGGGTGAGG(+ATATAT)GTACCAGTAAGACCCCGAGATTAGGTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240607035157_2t2e GTGACTCACTCTCAGTGGTACTGAGGAGGCAG(+ATATAT)GATCCTCATCTTCCTGTACTGCCCACCTGAC NGG 10 8 17 8 24 0 100 5 10
    240607035156_po9k AACCGGTCAGGTGGGCAGTACAGGAAGATGAG(+ATATAT)GATCCTGCCTCCTCAGTACCACTGAGAGTGA NGG 10 8 17 8 24 0 100 5 10