Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035112_h4hl TTTTTTGTCAATCATATGATTTTCTGAAGAAG(+ATATAT)GTACCACCATGCTATGGTGCTGTTTGAATCC NGG 10 8 17 8 24 0 100 5 10
    240607035111_izkq CCCATGGATTCAAACAGCACCATAGCATGGTG(+ATATAT)GTACCTTCTTCAGAAAATCATATGATTGACA NGG 10 8 17 8 24 0 100 5 10
    240607035110_611h AAGAAGAAAGCAAGACACAGGGCTGCACTTTG(+ATATAT)GATCCACGTTTAATGAAAAAGAAGTCACGTG NGG 10 8 17 8 24 0 100 5 10
    240607035108_990x CCAGTCACGTGACTTCTTTTTCATTAAACGTG(+ATATAT)GATCCAAAGTGCAGCCCTGTGTCTTGCTTTC NGG 10 8 17 8 24 0 100 5 10
    240607035107_c8dl CCCCTGGAGCTGGAGTTACAGGAGGTTGTGAG(+ATATAT)GTACCCGGTGTGGGTGCTGGGAGCCAAACTT NGG 10 8 17 8 24 0 100 5 10
    240607035105_6ag8 GACCCAAGTTTGGCTCCCAGCACCCACACCGG(+ATATAT)GTACCTCACAACCTCCTGTAACTCCAGCTCC NGG 10 8 17 8 24 0 100 5 10
    240607035104_bv3b TTATAACCTTCCAGCATTGGTTCTTGACTTTG(+ATATAT)GATCCAAAGCCCCAGTAGAAGAGAAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240607035102_6xq8 TCTGCTCCATCTTCTCTTCTACTGGGGCTTTG(+ATATAT)GATCCAAAGTCAAGAACCAATGCTGGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240607035101_yy7g GACCTCCAAAGGTACAGGTCAGAATAGGGCAG(+ATATAT)GATCCCTTGTGAACTACCCTACCATGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240607035059_c1ts TCAGCACCCACATGGTAGGGTAGTTCACAAGG(+ATATAT)GATCCTGCCCTATTCTGACCTGTACCTTTGG NGG 10 8 17 8 24 0 100 5 10