Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034956_rm2s TGTTAGCGAAACACCTTTAAAGATTTATAAGG(+ATATAT)GATCCCAGGTAATTAGTATATATGAAATTTT NGG 10 8 17 8 24 0 100 5 10
    240607034954_gdoc CAATAAAAATTTCATATATACTAATTACCTGG(+ATATAT)GATCCCTTATAAATCTTTAAAGGTGTTTCGC NGG 10 8 17 8 24 0 100 5 10
    240607034953_ddwt GTCTGAGAGCTCTGCACGGGCACAGATGCTGG(+ATATAT)GTACCTCTTATGGGTGTGGCAGTATTACACG NGG 10 8 17 8 24 0 100 5 10
    240607034951_pok3 TGTTTCGTGTAATACTGCCACACCCATAAGAG(+ATATAT)GTACCCAGCATCTGTGCCCGTGCAGAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240607034950_86rt GTCTGAGAGCTCTGCACGGGCACAGATGCTGG(+ATATAT)GTACCTCTTATGGGTGTGGCAGTATTACACG NGG 10 8 17 8 24 0 100 5 10
    240607034949_69uu TGTTTCGTGTAATACTGCCACACCCATAAGAG(+ATATAT)GTACCCAGCATCTGTGCCCGTGCAGAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240607034947_r9b0 TTCAAGCAACATATAAAATGAAGAGTTTCCTG(+ATATAT)GTACCTATCATAGTATTTACATTCCCTTATC NGG 10 8 17 8 24 0 100 5 10
    240607034946_ruvi TTTGAGATAAGGGAATGTAAATACTATGATAG(+ATATAT)GTACCAGGAAACTCTTCATTTTATATGTTGC NGG 10 8 17 8 24 0 100 5 10
    240607034944_4usi GGTGATCAACTTGACTTAAAATTGACCCTATG(+ATATAT)GATCCAAAAGATGATGATGCTAAACAGTATT NGG 10 8 17 8 24 0 100 5 10
    240607034943_isqt AAACTAATACTGTTTAGCATCATCATCTTTTG(+ATATAT)GATCCATAGGGTCAATTTTAAGTCAAGTTGA NGG 10 8 17 8 24 0 100 5 10