Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034927_w3x7 GAGAACTGTATCTCCCACCTGGAACTGGTGAG(+ATATAT)GTACCATCAAATCTCCTGGTTCGTAGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240607034926_6a4r TCAAGGAGGCCTACGAACCAGGAGATTTGATG(+ATATAT)GTACCTCACCAGTTCCAGGTGGGAGATACAG NGG 10 8 17 8 24 0 100 5 10
    240607034925_joy2 AAGGGTTACAGATAATAAAGAAATCACAAGTG(+ATATAT)GTACCTTTCTAAGAATTGCATATTTTTTTAG NGG 10 8 17 8 24 0 100 5 10
    240607034924_fwz4 AATTCTAAGAAAGCTGTGAGTTATGCAAGATG(+ATATAT)GTACCTTGGGGTTTTTGAATCAGGGGGATCA NGG 10 8 17 8 24 0 100 5 10
    240607034921_qxfw GATCCTGATCCCCCTGATTCAAAAACCCCAAG(+ATATAT)GTACCATCTTGCATAACTCACAGCTTTCTTA NGG 10 8 17 8 24 0 100 5 10
    240607034920_k69e GTGTGTGGTGCTAGCATGCTAGACAAAGCACG(+ATATAT)GTACCACTTTACAACATTGCAGCCCCGTGTT NGG 10 8 17 8 24 0 100 5 10
    240607034918_mr8x TCTGGAACACGGGGCTGCAATGTTGTAAAGTG(+ATATAT)GTACCGTGCTTTGTCTAGCATGCTAGCACCA NGG 10 8 17 8 24 0 100 5 10
    240607034917_qzsl TTTGCTCTTTCAGGTGGAGGCTTCGTTTCTGG(+ATATAT)GATCCCACTGCACCGCCATTTCTTCCCAGAT NGG 10 8 17 8 24 0 100 5 10
    240607034915_j82c CATAAATCTGGGAAGAAATGGCGGTGCAGTGG(+ATATAT)GATCCCAGAAACGAAGCCTCCACCTGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607034914_lfme GAAAAACAATGACTGGAATATCATTGGAGATG(+ATATAT)GATCCCAGGTAGGGGTTACAATTAGCCATGG NGG 10 8 17 8 24 0 100 5 10