Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034826_7kj9 AACACAGTTTGTGTGAGTTGTCCACCAACCAG(+ATATAT)GTACCATTATAAAGTGTCGTCATCTGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240607034825_xnl6 CCAGCTGGCTGGATGTGCCCAGATCCTCCTTG(+ATATAT)GTACCCTGTCCCTTTTCCTACAATGATTTTT NGG 10 8 17 8 24 0 100 5 10
    240607034823_ejj6 CATAGAAAAATCATTGTAGGAAAAGGGACAGG(+ATATAT)GTACCAAGGAGGATCTGGGCACATCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607034822_p0h3 AATGAGTATCATAGATGGTCAGGCTGCAGATG(+ATATAT)GATCCCAGAACCTGCAGCTACCACGGACACC NGG 10 8 17 8 24 0 100 5 10
    240607034820_bvi2 CTCCTGGTGTCCGTGGTAGCTGCAGGTTCTGG(+ATATAT)GATCCATCTGCAGCCTGACCATCTATGATAC NGG 10 8 17 8 24 0 100 5 10
    240607034819_02y9 AATGACCCTCACTTAGGAAGCAGTCATGATAG(+ATATAT)GTACCCAAACTTTTAAACCAGGCAGGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240607034817_mt6o TCACTGTGCTCCTGCCTGGTTTAAAAGTTTGG(+ATATAT)GTACCTATCATGACTGCTTCCTAAGTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240607034816_8s42 GCAGACATCTCTACCTCTTAACCCAGAGTTCG(+ATATAT)GATCCCTCGGACTTCCAAAATCACACCTCTG NGG 10 8 17 8 24 0 100 5 10
    240607034813_xfau TTCCACAGAGGTGTGATTTTGGAAGTCCGAGG(+ATATAT)GATCCGAACTCTGGGTTAAGAGGTAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240607034812_vkzh GGTTTGGTAAAGACATGTGACCAGGCATAAAG(+ATATAT)GATCCTTTCGCTGAGCCTTTAGAAAGGCATT NGG 10 8 17 8 24 0 100 5 10