Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034202_szn3 TCTACAAAGGGATGGACAGGAGAGTGCAACAG(+ATATAT)GATCCGGTTTATTCTCCTTGGCAAGGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240607034200_lwxl CTGCTGGCATTGGGCCTCTGAGGCGGGGTTGG(+ATATAT)GTACCTTCCTCCTTGGGCCTCATACTACTTA NGG 10 8 17 8 24 0 100 5 10
    240607034158_5cwn AAAACTAAGTAGTATGAGGCCCAAGGAGGAAG(+ATATAT)GTACCCAACCCCGCCTCAGAGGCCCAATGCC NGG 10 8 17 8 24 0 100 5 10
    240607034156_b65t TACATGCCTCCCAGTCCCTCCACAGCAACCGG(+ATATAT)GTACCTGGGGATTTTTCCCAAAGGACTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607034153_3kqr TGGCAATGGAGTCCTTTGGGAAAAATCCCCAG(+ATATAT)GTACCCGGTTGCTGTGGAGGGACTGGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240607034152_qaur GGGGCATGCTGGCTTTTCTCTGCCACACTCTG(+ATATAT)GATCCCTGTGCTCTGCTTGCTGGTGAATGAC NGG 10 8 17 8 24 0 100 5 10
    240607034151_vz7x CAGTGGTCATTCACCAGCAAGCAGAGCACAGG(+ATATAT)GATCCAGAGTGTGGCAGAGAAAAGCCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240607034149_jr0g TTATACAGCCCAGACCTACCTGCCTAGGGATG(+ATATAT)GTACCACCCGAAGATAGCTACATCAATCAAG NGG 10 8 17 8 24 0 100 5 10
    240607034148_z5mn CTTTTCTTGATTGATGTAGCTATCTTCGGGTG(+ATATAT)GTACCATCCCTAGGCAGGTAGGTCTGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240607034146_lj1c AGTCAGAAAGAACTCCATCACGAGGTAAGGAG(+ATATAT)GTACCGTGTCCAGGAGCTTGCTCCACAGTTC NGG 10 8 17 8 24 0 100 5 10