Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034249_pdm9 TGAATCTCAGAGGCTTGGGCAACAGGTAAGAG(+ATATAT)GATCCAAGGTGAAAATATGAGCCAACAGCAC NGG 10 8 17 8 24 0 100 5 10
    240607034247_9j9r CCCAATAGTCCTTTCCACTTTAACTCTTCCAG(+ATATAT)GTACCTTTTGGTCCTGCTGTAGCCACTGGCA NGG 10 8 17 8 24 0 100 5 10
    240607034246_2ze5 GGGCATGCCAGTGGCTACAGCAGGACCAAAAG(+ATATAT)GTACCTGGAAGAGTTAAAGTGGAAAGGACTA NGG 10 8 17 8 24 0 100 5 10
    240607034245_1uyp GAGGACAGAGGACAACTCAGAGTATTCTTCAG(+ATATAT)GTACCCTTCATTTTTCTCACTGGCCCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240607034243_ymp3 GGCGAGCCCTGGGCCAGTGAGAAAAATGAAGG(+ATATAT)GTACCTGAAGAATACTCTGAGTTGTCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240607034241_3tas CTCCCTCTTACCAACCATATGCATACGCCAAG(+ATATAT)GATCCTGTCCCTCTAGGTCTGTCAGCTTGCA NGG 10 8 17 8 24 0 100 5 10
    240607034240_l4y1 GAGCATGCAAGCTGACAGACCTAGAGGGACAG(+ATATAT)GATCCTTGGCGTATGCATATGGTTGGTAAGA NGG 10 8 17 8 24 0 100 5 10
    240607034238_qwq6 GCTTGCCCAGTCCCTGCACGGTAGATCCCTTG(+ATATAT)GTACCTGCAGCTCTCAGGTACGGAAGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240607034237_tv3k TAGACCTTTCCTTCCGTACCTGAGAGCTGCAG(+ATATAT)GTACCAAGGGATCTACCGTGCAGGGACTGGG NGG 10 8 17 8 24 0 100 5 10
    240607034235_ww3e GTATTTATCTGAGTATTTTACAAAGCACCTAG(+ATATAT)GTACCAAAAGTTAAAAATGTAAAACTCCAAG NGG 10 8 17 8 24 0 100 5 10