Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034234_9f4y TGTTGCTTGGAGTTTTACATTTTTAACTTTTG(+ATATAT)GTACCTAGGTGCTTTGTAAAATACTCAGATA NGG 10 8 17 8 24 0 100 5 10
    240607034233_a4si CAGTCCACAGGAAGCTTCCGGACATTATCTTG(+ATATAT)GTACCACATGAAGTCTGTAGGATGTATCCTC NGG 10 8 17 8 24 0 100 5 10
    240607034231_11fn CTTGAGAGGATACATCCTACAGACTTCATGTG(+ATATAT)GTACCAAGATAATGTCCGGAAGCTTCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240607034230_dix6 TTAAAACCTGATTCCAATGGAGTAAACACCTG(+ATATAT)GATCCCAACCGCCCATCTATGACAAACGGCT NGG 10 8 17 8 24 0 100 5 10
    240607034228_t8v8 TCCACAGCCGTTTGTCATAGATGGGCGGTTGG(+ATATAT)GATCCAGGTGTTTACTCCATTGGAATCAGGT NGG 10 8 17 8 24 0 100 5 10
    240607034226_lvpy TCTAGCAACCTTATTCTAACTACTGTGCCCTG(+ATATAT)GATCCACACAGGTTGGGGCTGCCACTGTGTA NGG 10 8 17 8 24 0 100 5 10
    240607034225_jkv9 TCAAGTACACAGTGGCAGCCCCAACCTGTGTG(+ATATAT)GATCCAGGGCACAGTAGTTAGAATAAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240607034224_jids TCCAGGAGGAAGGCAACCCCTCTAAGGCTCTG(+ATATAT)GTACCTACAGGGCCCCCACCTTGGGGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240607034222_qqpl TCCAGCACTCCCCCAAGGTGGGGGCCCTGTAG(+ATATAT)GTACCAGAGCCTTAGAGGGGTTGCCTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240607034219_l4ea GCCGGTTTCCGGGCTGGCCCCTCCTACCCCTG(+ATATAT)GATCCCAGACTGTCCTGGATTCCACCCTGGG NGG 10 8 17 8 24 0 100 5 10