Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034334_x33p CAGAATCCGCTGGCACAAAGGGGCTGTGTTCG(+ATATAT)GATCCGCTATTTCTATTGGCACCGAGTATCT NGG 10 8 17 8 24 0 100 5 10
    240607034333_mw6c AAGTTCCAGGCCAGCCTGCTCTACATGGTGAG(+ATATAT)GATCCTGTGTCTCAGAAACAAGCAAACAAGC NGG 10 8 17 8 24 0 100 5 10
    240607034331_8r23 TGTGTGCTTGTTTGCTTGTTTCTGAGACACAG(+ATATAT)GATCCTCACCATGTAGAGCAGGCTGGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240607034330_csc4 TCCTGCCATGCAGTCTACCTGTTTTGAAAATG(+ATATAT)GATCCACTCTTAACAGAGCCAATACCCATGA NGG 10 8 17 8 24 0 100 5 10
    240607034329_n3df ATATTTCATGGGTATTGGCTCTGTTAAGAGTG(+ATATAT)GATCCATTTTCAAAACAGGTAGACTGCATGG NGG 10 8 17 8 24 0 100 5 10
    240607034328_qjt5 GCAAAAACAAACAAAAAACAAACCCCTTTTTG(+ATATAT)GTACCTGCACTGCATCCCTCGGGTGGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607034327_o39l AGGCACTGCCCCACCCGAGGGATGCAGTGCAG(+ATATAT)GTACCAAAAAGGGGTTTGTTTTTTGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240607034325_qcml AGCCAGGGCCTGCAGACCTCTACACATCCTGG(+ATATAT)GATCCAGGAGGGCACAGGGAAGGCCAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240607034323_r0w4 CTGGACAACCTGGCCTTCCCTGTGCCCTCCTG(+ATATAT)GATCCCAGGATGTGTAGAGGTCTGCAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240607034322_jp3y TACTTGTCCTAGCATTCGGAAGAGGAAAGCAG(+ATATAT)GATCCCAGCAAATAAACCAGCCAGCCAAACA NGG 10 8 17 8 24 0 100 5 10