Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034435_1jpb CCAGGTTTGGAAGTTGAATGATCACCTTCTCG(+ATATAT)GATCCTCTGACCTCCTCCTTCCCCCACCCTG NGG 10 8 17 8 24 0 100 5 10
    240607034433_78y6 GGCCTAACAAGGTCACCCTCAGATCTTCCAAG(+ATATAT)GTACCCATGGGAACCATGGTCTAGCACAGTG NGG 10 8 17 8 24 0 100 5 10
    240607034431_965v AGAACCACTGTGCTAGACCATGGTTCCCATGG(+ATATAT)GTACCTTGGAAGATCTGAGGGTGACCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240607034429_4thg CATAGCCCAGGATGGACAGGAGTACGTGGGAG(+ATATAT)GTACCTCCAAACACAGAGGTCCATGCGTGGG NGG 10 8 17 8 24 0 100 5 10
    240607034428_93g9 GCACGCCCACGCATGGACCTCTGTGTTTGGAG(+ATATAT)GTACCTCCCACGTACTCCTGTCCATCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607034426_p2pr ATGACCATGCCGCTGACTGGCTCCACTCTGAG(+ATATAT)GATCCAGGGTCCTACCACTGCTGATGCTTCG NGG 10 8 17 8 24 0 100 5 10
    240607034424_r7rh CCTGTCGAAGCATCAGCAGTGGTAGGACCCTG(+ATATAT)GATCCTCAGAGTGGAGCCAGTCAGCGGCATG NGG 10 8 17 8 24 0 100 5 10
    240607034422_7ux3 ACATAATGGAGCGACACAAACTGAGCAGCCGG(+ATATAT)GATCCGTGCTGCCGCCCTAGCTCGGAGCACC NGG 10 8 17 8 24 0 100 5 10
    240607034421_2g1r AAGAGGGTGCTCCGAGCTAGGGCGGCAGCACG(+ATATAT)GATCCCGGCTGCTCAGTTTGTGTCGCTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607034419_oahi ACACGACGCTGCCTCTGCCTCTCCAGCACTGG(+ATATAT)GATCCCAAGCCAGGCCTTTCATGTGAGTGCT NGG 10 8 17 8 24 0 100 5 10