Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034348_ult9 AAAAGAAATCCAGGGTTCCGAAGTCCTTCTAG(+ATATAT)GTACCTCAGGAATGTATGATTTGGGAAGTAG NGG 10 8 17 8 24 0 100 5 10
    240607034346_nk05 TGCCACCCCTTTGTCAAGCAGACAGACCTTGG(+ATATAT)GATCCACCCGAAGGACAAGATACCAGAAGCT NGG 10 8 17 8 24 0 100 5 10
    240607034344_5lci CTTGAAGCTTCTGGTATCTTGTCCTTCGGGTG(+ATATAT)GATCCCAAGGTCTGTCTGCTTGACAAAGGGG NGG 10 8 17 8 24 0 100 5 10
    240607034343_hjqe CCCTCCATAAGGAAACAAGGAAAGACATCAGG(+ATATAT)GTACCTACTTCCTATGCACCGTGGACACAGC NGG 10 8 17 8 24 0 100 5 10
    240607034341_a25o GCTGGGCTGTGTCCACGGTGCATAGGAAGTAG(+ATATAT)GTACCCTGATGTCTTTCCTTGTTTCCTTATG NGG 10 8 17 8 24 0 100 5 10
    240607034340_dl5j TGAGAGATTTGATTGGCAGAAGGAATCAAGAG(+ATATAT)GATCCTGCATTTAGGATGCATAGATGGCTAG NGG 10 8 17 8 24 0 100 5 10
    240607034339_unxx CGTCACTAGCCATCTATGCATCCTAAATGCAG(+ATATAT)GATCCTCTTGATTCCTTCTGCCAATCAAATC NGG 10 8 17 8 24 0 100 5 10
    240607034337_579x GGTTGTCCTATGACTTCCCCACATGTACCATG(+ATATAT)GTACCCATACATGCACACAGTGTACAAACAC NGG 10 8 17 8 24 0 100 5 10
    240607034336_gpbt TTTCTGTGTTTGTACACTGTGTGCATGTATGG(+ATATAT)GTACCATGGTACATGTGGGGAAGTCATAGGA NGG 10 8 17 8 24 0 100 5 10
    240607034335_vjpo TCCTGAGATACTCGGTGCCAATAGAAATAGCG(+ATATAT)GATCCGAACACAGCCCCTTTGTGCCAGCGGA NGG 10 8 17 8 24 0 100 5 10