Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034402_rftr TTGCATCATACTGCTCAAAGAAACAATGTCAG(+ATATAT)GTACCAATAGAGATTAATATAACACATGACA NGG 10 8 17 8 24 0 100 5 10
    240607034401_7sf2 CTTATGACTTAGAATAATATTTTTTTCACATG(+ATATAT)GATCCACCAGGGTTAGTTTCAGAGTCAGTAC NGG 10 8 17 8 24 0 100 5 10
    240607034400_mi4e TTCCAGTACTGACTCTGAAACTAACCCTGGTG(+ATATAT)GATCCATGTGAAAAAAATATTATTCTAAGTC NGG 10 8 17 8 24 0 100 5 10
    240607034358_ynrd AGGCATGCTGTGTCTGAAGTGTTTAATAAAGG(+ATATAT)GATCCTTGTTACAGAACTTGTCTTCCGATGC NGG 10 8 17 8 24 0 100 5 10
    240607034357_hlef GGTGTGCATCGGAAGACAAGTTCTGTAACAAG(+ATATAT)GATCCCTTTATTAAACACTTCAGACACAGCA NGG 10 8 17 8 24 0 100 5 10
    240607034355_j4jf GTGTTTGTGAAGCCCGAACAGTGTGAGTCCGG(+ATATAT)GTACCGTGTGTACATACATATGTAGGAATAA NGG 10 8 17 8 24 0 100 5 10
    240607034354_knuy AAACGTTATTCCTACATATGTATGTACACACG(+ATATAT)GTACCCGGACTCACACTGTTCGGGCTTCACA NGG 10 8 17 8 24 0 100 5 10
    240607034352_rd1f ACCCAATATCTCCTACACCTAGTTGCAATAGG(+ATATAT)GTACCTGTGCCCTGGACTTTCTCCTTACATG NGG 10 8 17 8 24 0 100 5 10
    240607034351_ik2z TAACCCATGTAAGGAGAAAGTCCAGGGCACAG(+ATATAT)GTACCCTATTGCAACTAGGTGTAGGAGATAT NGG 10 8 17 8 24 0 100 5 10
    240607034349_mabb ATTGACTACTTCCCAAATCATACATTCCTGAG(+ATATAT)GTACCTAGAAGGACTTCGGAACCCTGGATTT NGG 10 8 17 8 24 0 100 5 10