Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034504_b1kl GGCCACCACCATCCATAACTCCAGCTCCAGGG(+ATATAT)GATCCAGTGCTGTCTTCTGACCTCTGAAGAC NGG 10 8 17 8 24 0 100 5 10
    240607034503_evgj AGATAGAAGCTCAACTTGACAAGATTCGTCTG(+ATATAT)GATCCCAGGGTAATGCAAGACAAACCCACAC NGG 10 8 17 8 24 0 100 5 10
    240607034500_44pn AAATGGTGTGGGTTTGTCTTGCATTACCCTGG(+ATATAT)GATCCAGACGAATCTTGTCAAGTTGAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240607034459_ckri CAGTGGCGAGGGATGGTCTCTAGTCTTCTCTG(+ATATAT)GTACCTCAGCATCCCCAAAGGCTCTTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240607034458_nsiw TGGAAGCCAAAAGAGCCTTTGGGGATGCTGAG(+ATATAT)GTACCAGAGAAGACTAGAGACCATCCCTCGC NGG 10 8 17 8 24 0 100 5 10
    240607034456_gmyb AGTTGAGTCAGGTGGCAGACAGTTTCCACCAG(+ATATAT)GATCCAAGCCTCTGCGTATGCAGGGATATAT NGG 10 8 17 8 24 0 100 5 10
    240607034455_mgpg AAGCCATATATCCCTGCATACGCAGAGGCTTG(+ATATAT)GATCCTGGTGGAAACTGTCTGCCACCTGACT NGG 10 8 17 8 24 0 100 5 10
    240607034453_bayu GGCCTTGGCTCTCACAGTTCGAGAGAACCAGG(+ATATAT)GATCCTCTCGAAAGATGCAACTGCCCAACAG NGG 10 8 17 8 24 0 100 5 10
    240607034452_v6bs TGCACCTGTTGGGCAGTTGCATCTTTCGAGAG(+ATATAT)GATCCCTGGTTCTCTCGAACTGTGAGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607034450_451s CTGCCACCTGGAACGTGGTGGGATTTGGTTTG(+ATATAT)GATCCAAATACTGAGACCCTAGGAAACTTCA NGG 10 8 17 8 24 0 100 5 10