Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034547_6sk4 CTCACTGTGCGACTGCTGGGTCACTGGTGCAG(+ATATAT)GTACCGAGGATTTCTGGCTGGCTGGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240607034546_rehy AACATTCCAGTTTCCCACAGTCTTATCTTTGG(+ATATAT)GTACCGTTTACAAACTCATGAGGTTGACCTC NGG 10 8 17 8 24 0 100 5 10
    240607034545_i65i TTACAGAGGTCAACCTCATGAGTTTGTAAACG(+ATATAT)GTACCCAAAGATAAGACTGTGGGAAACTGGA NGG 10 8 17 8 24 0 100 5 10
    240607034542_x4f7 CAGAAAGATGGAAACGATCAGGGACAGCCCAG(+ATATAT)GATCCCTGATGAGAACTCATGATTTTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240607034541_w46m AATGGCGCAAAAAATCATGAGTTCTCATCAGG(+ATATAT)GATCCTGGGCTGTCCCTGATCGTTTCCATCT NGG 10 8 17 8 24 0 100 5 10
    240607034539_4pqf CCCTAGTTTCTGCCCTCCACACATCATCAGTG(+ATATAT)GTACCTTAAGTCTTATTCCTGCCAAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607034538_kk84 GATCTTTCCTTTTGGCAGGAATAAGACTTAAG(+ATATAT)GTACCACTGATGATGTGTGGAGGGCAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240607034537_g1gx CTGTTGCCTGTGAAGGTCAGAAAAGGGCAGTG(+ATATAT)GATCCCATGAGGCTGGATTTATAGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240607034535_1hvm GCTCACAACCATCTATAAATCCAGCCTCATGG(+ATATAT)GATCCACTGCCCTTTTCTGACCTTCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240607034534_q83y ACCACATGGTGGCTCACAACCATCTGTAATGG(+ATATAT)GATCCGATGCCCTCTTCTGGTGATGCTATTT NGG 10 8 17 8 24 0 100 5 10